相关实验视频

Updated: Jun 7, 2025

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Screening for Functional Non-coding Genetic Variants Using Electrophoretic Mobility Shift Assay EMSA and DNA-affinity Precipitation Assay DAPA

Published on: August 21, 2016

人类ABCC11载体基因变异与骨皮肤微生物组功能基因组学的相互作用

Bruce R Stevens¹, Luiz F W Roesch²

¹Department of Physiology and Aging, College of Medicine, University of Florida, Gainesville, FL, 32610, USA. stevensb@ufl.edu.

Scientific reports

|November 14, 2024

概括

人类的ABCC11基因通过控制细菌气味产生的前体来影响下气味. 一个特定的基因变异 (C等位基因) 能够产生气味,而另一个 (TT等位基因) 通过阻止前体转移来防止气味.

科学领域:

微生物学微生物学
人类遗传学人类遗传学
生物化学生物化学

背景情况:

人的部微生物组和皮肤细胞形成一个共生关系,影响汗水气味 (化症).
ABCC11基因在决定汗水是否有气味方面发挥着至关重要的作用.
无味的汗水前体被皮肤细菌转化为恶臭的化合物.

研究的目的:

研究人类ABCC11基因变体与皮肤微生物组产生气味的能力之间的家族关联.
了解人类体臭产生背后的遗传和微生物机制.

主要方法:

对ABCC11单核酸多形态 (SNP) 的受试者的基因定型.
ABCC11单元类型与臂微生物组DNA测序和功能元基因组学之间的相关性.
对多代家族血统进行分析,以确定遗传模式.

主要成果:

在ABCC11变异和气味产生方面观察到孟德尔的自体递归遗传模式.
ABCC11的C等位基因与细菌PatB基因的存在有关,该基因负责气味生物合成.
具有同卵性TT等位基因 (ABCC11功能丧失) 的宿主缺乏PatB基因,并且不会产生气味.

结论:

功能性ABCC11 (C等位基因) 对于提供前体分子至关重要,这些前体分子塑造皮肤微生物组以产生气味.
该ABCC11基因和特定的皮肤细菌 (葡萄球菌) 相互作用,以确定人体气味.
这种互动提供了对人类进化和与人体气味相关的人口遗传学的见解.

更多相关视频

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

In Vivo Functional Study of Disease-associated Rare Human Variants Using Drosophila

Published on: August 20, 2019

相关概念视频

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

Exon Recombination

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes.
Exon shuffling follows “splice frame rules.” Each exon...