Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jun 7, 2025

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
08:30

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

12.9K

一个全面的人类胚胎参考工具,使用单细胞RNA测序数据.

Cheng Zhao1, Alvaro Plaza Reyes1,2, John Paul Schell1

  • 1Department of Clinical Science, Intervention and Technology, Karolinska Institutet, and Division of Obstetrics and Gynecology, Karolinska Universitetssjukhuset, Stockholm, Sweden.

Nature methods
|November 14, 2024
PubMed

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Fibroblast-driven collagen expansion and altered thymic medullary niches in 22q11.2 deletion syndrome.

Journal of human immunity·2026
Same author

The development and impact of an innovative graduate program to promote entry to physiology-related careers: the Master of Health Science in Medical Physiology.

Advances in physiology education·2026
Same author

A transgene-free, human peri-gastrulation embryo model presents trilaminar embryonic disc-, amnion- and yolk sac-like structures.

Nature cell biology·2026
Same author

A guide to using embedded ethics in human stem-cell-based embryo model research.

Nature cell biology·2026
Same author

A controllable human spinal cord model with full dorsoventral patterning.

Nature communications·2026
Same author

Human stem cell-based embryo models: innovation, ethics, and policy.

Human reproduction (Oxford, England)·2026
Same journal

RNAbpFlow: base pair-augmented SE(3) flow matching for conditional RNA 3D structure generation.

Nature methods·2026
Same journal

Spatio-DARLIN enables robust and efficient in situ lineage tracing in mice at single-cell resolution.

Nature methods·2026
Same journal

EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control.

Nature methods·2026
Same journal

Cloud-based microscope enables live neuroimaging for 24 h and beyond with worldwide access.

Nature methods·2026
Same journal

Deep molecular profiling in three dimensions.

Nature methods·2026
Same journal

3D pathology-guided microdissection.

Nature methods·2026
查看所有相关文章
概括
此摘要是机器生成的。

研究人员为早期发育创建了一个人类单细胞RNA测序参考数据集. 该工具有助于准确地进行基准测试和验证人类胚胎模型,防止对发育研究的误解.

科学领域:

  • 发展生物学 发展生物学
  • 干细胞生物学 干细胞生物学
  • 基因组学就是基因组学.

背景情况:

  • 来自干细胞的人类胚胎模型是研究早期发育的宝贵工具.
  • 评估这些模型对体内发育的忠实性至关重要.
  • 单细胞RNA测序 (scRNA-seq) 是此评估的一个关键技术.

研究的目的:

  • 为早期发育开发一个全面的,综合的人类scRNA-seq参考数据集.
  • 建立一个标准化资源,用于对比和验证人类胚胎模型的真实性.
  • 创建一个用于在新型数据集中注释细胞身份的预测工具.

主要方法:

  • 整合了六个已发表的人类scRNA-seq数据集 (zygote 到 gastrula).
  • 使用人类和非人类灵长类动物数据验证血统注释.
  • 开发使用统一多重近似和投影 (UMAP) 的预测工具.

主要成果:

  • 建立了一个统一的人类scRNA-seq参考数据集,涵盖了早期胚胎发生.
  • 创建了一个预测工具,可以对查询数据集进行投影和注释.
  • 分析显示,现有的胚胎模型缺乏适当的基准测试,存在错误注释的风险.

更多相关视频

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
09:14

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

772
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

11.5K

相关实验视频

Last Updated: Jun 7, 2025

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
08:30

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

12.9K
Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution
09:14

Author Spotlight: A Pipeline to Analyze Lineage-Specific Mutant Embryos at Single-Cell Resolution

Published on: June 14, 2024

772
Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

11.5K

结论:

  • 开发的参考数据集和预测工具对于准确验证人类胚胎模型至关重要.
  • 使用此资源可以减轻错误注释的风险,并提高发展研究的可靠性.
  • 这项工作为评估基于干细胞的人类胚胎模型提供了通用基准.