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多种SARS-CoV-2变种的病毒形态和病毒上尖端蛋白质结构

Zunlong Ke^1,2,3, Thomas P Peacock^4,5, Jonathan C Brown⁴

¹Department of Cell and Virus Structure, Max Planck Institute of Biochemistry, Martinsried, Germany.

The EMBO journal

|November 15, 2024

概括

新的研究表明,SARS-CoV-2变种,包括阿尔法和三角形,在它们的尖端蛋白中表现出结构变化. 这些突变改变了当地的蛋白质结构,可能会影响病毒.

科学领域:

病毒学病毒学
结构生物学结构生物学
分子生物学分子生物学

背景情况:

严重急性呼吸系统综合征冠状病毒2 (SARS-CoV-2) 变种随着身体健康的提高而演变.
尖 (S) 蛋白是适应性免疫反应的关键标,并且经历了结构性适应.
之前的研究使用单粒子冷电子显微镜 (cryo-EM) 对可溶性S蛋白进行研究.

研究的目的:

为了分析SARS-CoV-2尖端蛋白在多个变体的完整病毒体上的现场结构.
评估S蛋白分裂,病毒形态,S结合和构造状态.
了解B.1,Alpha,Beta,Gamma,Delta,Kappa和Mu等变体的S蛋白中的氨基酸变化如何影响结构和动态.

主要方法:

使用了冷电子显微镜 (cryo-EM) 和冷电子断层扫描 (cryo-ET).
分析的重点是SARS-CoV-2变种B.1,Alpha,Beta,Gamma,Delta,Kappa和Mu的完整病毒.
评估的关键参数包括S蛋白分裂,病毒形态,S结合和in-situ S结构.

主要成果:

在研究的变种中,在总体的病毒形态学中没有观察到适应性变化.
在S蛋白内的多个位置显示了氨基酸变化,导致局部蛋白质结构发生了变化.
这项研究确定了一系列S蛋白形状状态在现场.

结论:

在SARS-CoV-2尖端蛋白中氨基酸替代导致局部结构变化.
这些结构变化,从尖的顶部到底部发生,可以调节S蛋白的结构动态.
现场冷EM分析为S结构及其在病毒进化中的作用提供了功能相关的见解.

关键词:

新冠病毒新冠病毒新冠病毒.低温电子断层扫描 (Cryo-electron Tomography) 是一种电子断层扫描技术.膜融合蛋白质是一种病毒的演化病毒的演化病毒结构病毒结构

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