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乳酸对牛体外受精和胚胎发育的影响
Bo-Myeong Kim1, Song-Hee Lee1, Geun Heo1
1Department of Animal Science, Chungbuk National University, Cheongju, Korea.
Animal bioscience
|November 15, 2024
概括
在Tyrode的albumin乳酸硫酸盐 (TALP) 介质中补充酸盐显著改善了牛体外受精和胚胎发育. 乳酸的4.5mM度通过调节氧化应激来增强胚胎细胞形成和胚胎质量.
科学领域:
- 生殖生物学 生殖生物学
- 动物科学动物科学
- 生物化学 生物化学
背景情况:
- 对于牛胚胎移植的需求需要在体外受精 (IVF) 技术的进步.
- 优化受精和胚胎发育媒介对于提高试管婴儿成功率至关重要.
研究的目的:
- 为了评估替换Tyrode的白蛋白乳酸硫酸盐 (TALP) 介质成分以乳酸对牛受精和胚胎发育的影响.
- 确定乳酸的最佳度,以提高试管婴儿的结果.
主要方法:
- 乳酸 (2.0,3.0,4.5mM) 在TALP介质中取代了乳酸和CaCl2用于受精.
- 实验2比较了4.5mM的乳酸与使用修改的乳酸和CaCl2度的对照组.
- 胚胎发育被评估到第8天后授精.
主要成果:
- 4.5mM的乳酸度显著增加了受精和胚胎细胞形成率.
- 这种度对胚胎细胞直径和细胞数量产生了积极的影响,同时降低了活性氧物种 (ROS) 和增加了谷氨 (GSH) 水平.
- 实验2通过4.5毫米的乳酸证实了增强的受精率和胚胎细胞率,以及改善的胚胎质量标志物 (减少ROS,增加GSH).
结论:
- 用乳酸代替TALP介质的成分有效地提高了牛的受精和胚胎发育.
- 乳酸的4.5毫米度是改善试管婴儿结果和胚胎质量的最佳水平,不管其他中间成分度如何.
- 这项研究引入了一种新的TALP介质配方,在牛胚胎产业中具有潜在的应用.
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