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在肝细胞癌中识别和验证Hsa_circ_0001726/miR-140-3p/KRAS轴基于微阵列分析和实验

Xiaobin Chi¹, Zhijian Chen¹, Jianda Yu¹

¹Department of Hepatobiliary Surgery, 900TH Hospital of Joint Logistics Support Force, Fuzhou, Fujian, China.

Journal of clinical and translational hepatology

|November 15, 2024

概括

这项研究揭示了涉及肝细胞癌 (HCC) 进展的循环RNA,微RNA和信使RNA网络. 在hsa_circ_0001726/miR-140-3p/KRAS轴促进HCC和lenvatinib的耐药性.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

肝细胞癌 (HCC) 是一种高度致命的恶性瘤.
非编码RNAs,包括microRNAs (miRNAs) 和循环RNAs (circRNAs),通过表观遗传调节在HCC病变发生中发挥关键作用.
了解这些调节网络对于开发新型治疗策略至关重要.

研究的目的:

在HCC中构建一个全面的circRNA-miRNA-mRNA调节网络.
确定推动HCC进展和药物耐药性的关键分子轴.
为了验证特定轴的功能作用,在HCC中hsa_circ_0001726/miR-140-3p/KRAS.

主要方法:

利用基因表达总线 (GEO) 用于HCC转录组数据和基因识别的GeneCards.
预测circRNA-miRNA和miRNA-mRNA相互作用以建立一个调节网络.
进行了体外细胞实验 (增殖,迁移) 和生存分析,以评估已识别的轴.

主要成果:

构建了一个由6个差异表达的circRNA,13个miRNA和88个mRNA组成的网络.
鉴定并验证了hsa_circ_0001726/miR-140-3p/KRAS轴促进HCC细胞的增殖和迁移.
hsa_circ_0001726在HCC患者的整体存活期和介导伦瓦替尼布耐药性方面显示出预后价值.

结论:

开发的circRNA/miRNA/mRNA网络为HCC转录后调节提供了新的见解.
hsa_circ_0001726/miR-140-3p/KRAS轴与HCC进展和伦瓦提尼布耐药性有关,代表了一个潜在的治疗点.

关键词:

生物信息学是一种生物信息学.这是循环RNARNA.肝细胞癌是一种肝细胞癌.预测预测预测预测铁氨酸激酶抑制剂铁氨酸激酶抑制剂这是一个微型RNA.

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