使用VSWIR光谱学在冬季小麦中进行宏观营养素和微量营养素分析
Anmol Kaur Gill1, Srishti Gaur1, Clay Sneller2
1Department of Food, Agricultural, and Biological Engineering, Ohio State University, Columbus, OH, United States.
Frontiers in plant science
|November 15, 2024
概括
可见到短波红外 (VSWIR) 反射率准确地预测冬季小麦叶子的营养和水分. 这种高通量方法可以为精准农业和育种计划进行快速,非破坏性的现场评估.
科学领域:
- 农业科学 农业科学
- 植物生理学 植物生理学
- 遥感 遥感 遥感 遥感
背景情况:
- 精确量化叶子营养素对于优化作物健康和冬季小麦产量至关重要.
- 传统的营养分析方法往往是破坏性的,耗时的,不适合大规模的现场评估.
- 可见到短波红外光谱 (VSWIR) 为评估植物生理状态提供了一种潜在的非破坏性方法.
研究的目的:
- 评估叶子级VSWIR反射在预测冬季小麦中宏观营养素,微量营养素和水分度方面的有效性.
- 开发和验证使用部分最小平方回归 (PLSR) 的预测模型,并探索不同的组件选择方法.
- 评估VSWIR光谱在小麦育种和精准农业中进行高通量,非破坏性营养分析的潜力.
主要方法:
- 在两个生长季节收集了360个冬季小麦叶样本,用于VSWIR反射度测量 (3502,500nm) 和化学分析.
- 开发了使用部分最小平方回归 (PLSR) 的预测模型,并采用了三个组件选择方法:绝对最小的PRESS,对PRESS的向后代和Van der Voet的随机t-test.
- 通过交叉验证评估模型性能,并通过预测 (VIP) 评分中的变量重要性检查光谱区域的重要性.
主要成果:
- 逆向代方法通常在模型性能和复杂性之间提供了良好的平衡.
- 对于一些营养和水分含量,包括 (0.84), (0.75), (0.78),水分 (0.84) 等,可以达到很高的预测准确度 (R2值).
- 变量重要性分析确定了对模型预测能力有贡献的关键光谱区域.
结论:
- 叶子级VSWIR反射率与PLSR相结合,是一种强大的高通量方法,用于量化冬季小麦中的多种叶子营养素和水分.
- 这种非破坏性的方法促进了快速而精确的现场评估,支持精准农业,提高了小麦育种计划的效率.
- 这项研究证明了VSWIR光谱技术在解决营养缺乏和优化作物管理策略方面的潜力.
相关概念视频
01:15High-Resolution Mass Spectrometry (HRMS)
The resolution of a mass spectrometer depends on the efficiency of separating ions with different ion masses. The mass of an atom is approximated to the sum of the masses of protons and neutrons inside, considering the masses of protons and neutrons as equal. However, the masses of the proton (1.6726 × 10−24 g) and neutron (1.6749 × 10−24 g) are not truly equal. There is a minor error in the expression of atomic masses relative to the simplest atom of hydrogen. For example, the mass of helium...
01:14UV–Vis Spectrometers
The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...
01:29UV–Vis Spectroscopy: Woodward–Fieser Rules
UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π → π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the contributions...
01:11Applications of IR Spectroscopy: Overview
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...