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精子质量分析仪:便携式LED阵列显微镜,具有暗场成像功能
Meng Shao1, Changxu Li1, Xiaohao Ma1
1School of Biomedical Engineering Anhui Medical University Hefei China.
Bioengineering & translational medicine
|November 15, 2024
概括
使用编码的LED照明和暗场成像的便携式低成本显微镜能够准确分析精子质量. 该系统与LabCASA软件一起,为护理点测试提供了具有成本效益的解决方案,增强了不孕症诊断.
科学领域:
- 生物医学工程 生物医学工程
- 生殖生物学 生殖生物学
- 显微镜的使用方法
背景情况:
- 精子质量分析对于不孕症诊断至关重要,通常使用昂贵且庞大的计算机辅助精子分析 (CASA) 系统.
- 现有的CASA系统面临着在医疗点检测 (POCT) 的成本和可移植性方面的挑战.
研究的目的:
- 开发一种便宜的便携式显微镜,用于精子质量分析,适合POCT.
- 创建一个与便携式显微镜兼容的CASA软件,用于评估精子运动.
主要方法:
- 使用编码发光二极管 (LED) 阵列照明用于多对比成像.
- 开发了一种便携式显微镜 (16.5 × 14.0 × 25.0厘米) 用于暗场 (DF) 成像.
- 创建了LabCASA软件,用于使用DF成像数据进行精子运动分析.
主要成果:
- 携带DF成像的便携式显微镜提供了适合CASA的高对比精子图像.
- 与TrackMate相比,LabCASA软件显示出高可靠性,运动参数差异<10% CV.
- 该系统在跟踪精子运动方面表现出强度,并证实了运动参数随温度变化的变化.
结论:
- 开发的便携式显微镜系统与DF成像和LabCASA软件是精子质量分析的经济有效解决方案.
- 该系统非常适合用于精子质量的便携式POCT,特别是在资源有限的环境中.
- 暗场成像方法提高了精子运动性评估的稳定性和准确性.
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