Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Single-Atom Migration into a Chiral Multilayer Nanocluster Architecture.

Journal of the American Chemical Society·2026
Same author

Multimodal analytical strategy for revealing the related compounds of color variation in Salvia miltiorrhiza after sweating.

Journal of pharmaceutical and biomedical analysis·2026
Same author

Saturated alginate oligosaccharide as a new functional food: preparation, characterization and alleviation of cognitive impairment through pre-intervention.

Food & function·2026
Same author

Phenylalanine metabolism as a key pathway in polystyrene microplastics-induced abnormal macrophage polarization: Implications for fetal growth and development.

Chemico-biological interactions·2026
Same author

Label-Free Detection of Acrosome Reaction of Human Sperm Based on Diffraction Phase Microscopy.

Chemical & biomedical imaging·2026
Same author

Man With Sudden Onset Dyspnea.

Annals of emergency medicine·2026

相关实验视频

Updated: Jun 7, 2025

Techniques for Imaging Ca2+ Signaling in Human Sperm
07:38

Techniques for Imaging Ca2+ Signaling in Human Sperm

Published on: June 16, 2010

17.1K

精子质量分析仪:便携式LED阵列显微镜,具有暗场成像功能.

Meng Shao1, Changxu Li1, Xiaohao Ma1

  • 1School of Biomedical Engineering Anhui Medical University Hefei China.

Bioengineering & translational medicine
|November 15, 2024
PubMed
概括
此摘要是机器生成的。

使用编码的LED照明和暗场成像的便携式低成本显微镜能够准确分析精子质量. 该系统与LabCASA软件一起,为护理点测试提供了具有成本效益的解决方案,增强了不孕症诊断.

关键词:
在LED阵列显微镜中使用LED.在 LabCASASA 工作室.暗场成像技术使用暗场成像.精子运动性 精子运动性

更多相关视频

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
19:26

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

Published on: May 24, 2013

20.2K
Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm
05:04

Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm

Published on: October 13, 2023

959

相关实验视频

Last Updated: Jun 7, 2025

Techniques for Imaging Ca2+ Signaling in Human Sperm
07:38

Techniques for Imaging Ca2+ Signaling in Human Sperm

Published on: June 16, 2010

17.1K
Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging
19:26

Measuring Intracellular Ca2+ Changes in Human Sperm using Four Techniques: Conventional Fluorometry, Stopped Flow Fluorometry, Flow Cytometry and Single Cell Imaging

Published on: May 24, 2013

20.2K
Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm
05:04

Author Spotlight: A Live Cell Imaging Technique to Study Calcium Signaling and Acrosome Exocytosis in Mouse Sperm

Published on: October 13, 2023

959

科学领域:

  • 生物医学工程 生物医学工程
  • 生殖生物学 生殖生物学
  • 显微镜的使用方法

背景情况:

  • 精子质量分析对于不孕症诊断至关重要,通常使用昂贵且庞大的计算机辅助精子分析 (CASA) 系统.
  • 现有的CASA系统面临着在医疗点检测 (POCT) 的成本和可移植性方面的挑战.

研究的目的:

  • 开发一种便宜的便携式显微镜,用于精子质量分析,适合POCT.
  • 创建一个与便携式显微镜兼容的CASA软件,用于评估精子运动.

主要方法:

  • 使用编码发光二极管 (LED) 阵列照明用于多对比成像.
  • 开发了一种便携式显微镜 (16.5 × 14.0 × 25.0厘米) 用于暗场 (DF) 成像.
  • 创建了LabCASA软件,用于使用DF成像数据进行精子运动分析.

主要成果:

  • 携带DF成像的便携式显微镜提供了适合CASA的高对比精子图像.
  • 与TrackMate相比,LabCASA软件显示出高可靠性,运动参数差异<10% CV.
  • 该系统在跟踪精子运动方面表现出强度,并证实了运动参数随温度变化的变化.

结论:

  • 开发的便携式显微镜系统与DF成像和LabCASA软件是精子质量分析的经济有效解决方案.
  • 该系统非常适合用于精子质量的便携式POCT,特别是在资源有限的环境中.
  • 暗场成像方法提高了精子运动性评估的稳定性和准确性.