铜对大鼠囊腺里林分泌的调节作用
Rui Sun1,2,3, Zhongshen Wang1,2,3, Meng Li1,2,3
1College of Animal Science and Technology, Jilin Agricultural University, Changchun, Jilin Province, China.
Journal of animal physiology and animal nutrition
|November 15, 2024
概括
铜补充剂通过调节关键激素格林来增强生长. 这项研究显示,铜通过JNK/p38 MAPK通路和AP-1激活来增加林的合成和分泌,同时抑制体静止素.
科学领域:
- 动物营养和新陈代谢
- 内分泌学 在内分泌学.
- 分子生物学分子生物学
背景情况:
- 铜 (Cu) 是促进动物生长的重要料添加剂.
- 增长激素 (GH) 轴,包括 ghrelin,在增长调节中起着至关重要的作用.
- 铜影响格林介导成长的确切机制尚不清楚.
研究的目的:
- 为了研究铜如何影响 ghrelin 的合成和分泌.
- 阐明参与铜介导 ghrelin 调节的分子通路.
- 了解体静止素 (SS) 在铜对 ghrelin 的影响中的作用.
主要方法:
- 斯普拉格 - 达利大鼠被食不同度硫酸铜 (0.120,240毫克/公斤) 的饮食.
- 血清和胃组织分析使用ELISA,qRT-PCR和西部血栓检测进行.
- 关键的信号分子,包括格林,GOAT,AG,GH,SS,ROS,JNK,p38 MAPK,AP-1及其受体,被量化和分析.
主要成果:
- 一种120 mg/kg的铜补充剂改善了生长性能.
- 铜显著增加了血清林,GOAT,AG,GH和ROS水平,同时降低了SS.
- 铜高调格林,GOAT,GHS-R1α和AP-1表达,激活的JNK/p38 MAPK,以及低调的SS/SSTR2表达.
结论:
- 铜通过ROS/JNK/p38 MAPK/AP-1通路转录影响胃林合成.
- 索马托斯坦在铜介导的 ghrelin 调节中起着至关重要的作用.
- 铜通过增强GOAT活性和抑制SS分泌来促进林分泌和化,影响林/GOAT/GHS-R1α系统.
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