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人工级联转化生物系统用于通过更明智的糖利用对奇数的新糖糖和d-糖进行单生物制造

Xiaohan Xu¹, Jing Gao¹, Liwei Qing¹

¹State Key Laboratory of Marine Food Processing and Safety Control, College of Food Science and Engineering, Ocean University of China, Qingdao 266404, PR China.

Journal of agricultural and food chemistry

|November 15, 2024

概括

研究人员开发了一种新的三种酶系统,用于从agarose中产生新agarotriose (NA3). 这种方法还将副产品转化为有价值的d-塔加,提供一种更有效的利用阿加的方法.

科学领域:

生物技术是生物技术.
酶学是一种酶学.
碳水化合物化学碳水化合物化学

背景情况:

糖寡糖具有多样化的生物活动,但针对性生产特定结构,如奇数新糖寡糖 (NAOSs) 是具有挑战性的.
在NAOS生产的一个关键限制是缺乏高效的一步化酶的目标合成,特别是对于新酸 (NA3) 等化合物.

研究的目的:

开发一种新型的酶级联系统,用于从阿加中准生产新阿加托里 (NA3).
为了将NA3合成过程中产生的低价值副产品提升为一种有价值的化合物,d-tagatose.
通过酶转化建立一个高效和更明智的应用路径,通过酶转化利用阿加洛斯.

主要方法:

协同应用α-agarase (AgaA33) 和β-galactosidase (BgaD) 来从糖中产生NA3.
来自*Clostridium acetobutylicum*的L-阿拉比诺酸异合酶 (CaLAI) 的表征,用于将d-银糖转化为d-糖.
对三种酶级联系统的反应条件的优化,以最大限度地提高NA3和d-塔加的生产.

主要成果:

这项研究成功地使用协同酶的方法准备了NA3,并使用了亚加作为基质.
鉴定了L-阿拉比诺酸异构酶 (CaLAI) 的特征,并证明了d-银糖转化为d-塔加的有效转化,具有良好的热稳定性,不需要金属离子.
在最佳条件下,三种酶级联系统实现了NA3的产量为0.40 g/L,而d-塔加的产量为0.15 g/L,这是迄今为止最高的NA3产量.

结论:

开发的人工三酶级联系统代表了NA3.3有针对性的生产的重大进步.
这种系统有效地将d-银酸的副产品提升为d-银酸,提高了阿加使用的整体经济可行性.
这项研究提供了一个有前途且高效的酶策略,用于全面应用糖资源.

关键词:

这是一种阿加洛斯寡糖类糖.综合利用综合利用 d-塔加托糖的使用.多种酶级联级联.新阿加洛特里奥斯 (NeoAgarotriose) 是一种新兴的植物.

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