深度欧性溶剂辅助乳糖酶预处理,以改善玉米炉的酶性水解
Kexin Lin1, Weiting Zhang1, Xinyang Fan1
1College of Life Sciences, Northeast Forestry University, No. 26, Hexing Road, Harbin, 150040, China.
Bioprocess and biosystems engineering
|November 15, 2024
概括
将乳糖酶与深度性溶剂 (DES) 结合起来,可以有效地从玉米中去除红素. 这种预处理显著提高了用于生物乙醇生产的可发酵糖产量.
科学领域:
- 生物技术是生物技术.
- 生物质预处理 生物质预处理
- 酶工程是什么? 酶工程是什么?
背景情况:
- 素的去除对于有效地从基纤维素生物质生产生物乙醇至关重要.
- 深度环氧化溶剂 (DES) 为生物质加工提供了一个有前途的绿色化学方法.
- 乳酶是能够降解红素的酶,但它们的应用需要优化.
研究的目的:
- 为了研究基于laccase和贝他因的DES在玉米炉前处理中的协同效应.
- 评估DESs对乳糖酶活性,稳定性和素降解能力的影响.
- 增强用于生物乙醇应用的降解糖的生产.
主要方法:
- 制备和描述三种基于贝他因的DESs.
- 在水性DES溶液中评估laccase活性和热稳定性.
- 使用LC-MS.的lignin模型化合物降解分析.
- 玉米炉的预处理,使用乳糖酶和DES的组合.
主要成果:
- 基于贝他因的DESs没有抑制乳糖酶的活性,并且增强了它的热稳定性.
- 在70°C的DES中,laccase在一个小时内保持了95%以上的活性.
- 乳糖酶通过裂解β-O-4键,成功降解了一种素模型化合物.
- 乳糖酶和贝他因糖醇DES的组合实现了最大的素去除.
- 与对照组相比,这种预处理策略使发酵糖的产量增加了130%.
结论:
- 与基于贝他因的DES集成的Laccase是一种有效和稳定的林氏纤维素生物质预处理系统.
- 这种方法显著改善了木质素的去除,并提高了可发酵糖的产量.
- 这些发现为在工业生物乙醇生产中应用laccase-DES系统提供了基础.
相关概念视频
01:13Esters to Carboxylic Acids: Acid-Catalyzed Hydrolysis
2.7K
Hydrolysis of esters under acidic conditions proceeds through a nucleophilic acyl substitution. In the presence of excess water, the reaction proceeds in a reversible manner, forming carboxylic acids and alcohols.
During hydrolysis, the ester is first activated towards nucleophilic attack through the protonation of the carboxyl oxygen atom by the acid catalyst. The protonation makes the ester carbonyl carbon more electrophilic. In the next step, water acts as a nucleophile and adds to the...
During hydrolysis, the ester is first activated towards nucleophilic attack through the protonation of the carboxyl oxygen atom by the acid catalyst. The protonation makes the ester carbonyl carbon more electrophilic. In the next step, water acts as a nucleophile and adds to the...
2.7K
01:08Sample Preparation for Analysis: Advanced Techniques
301
Accurate analysis of complex samples often requires advanced preparation techniques to achieve reliable and reproducible results. Samples containing inorganic or organic materials can be challenging to dissolve or decompose effectively. Standard sample preparation methods include acid digestion, fusion, dry ashing, and wet digestion.
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
Acid digestion with strong acids is commonly used to dissolve inorganic materials that are insoluble (do not dissolve) in water. This method can be useful for...
301