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阿迪波涅丁对子中氧化应激诱导的卵巢颗粒细胞衰老的保护作用

Yan Zheng¹, Yunqiao Qiu¹, Ming Gao¹

¹Key Laboratory of Livestock Infectious Diseases, Ministry of Education, and Key Laboratory of Ruminant Infectious Disease Prevention and Control (East), Ministry of Agriculture and Rural Affairs, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.

Poultry science

|November 15, 2024

概括

素 (ADPN) 降低了粒细胞 (GCs) 中的氧化应激和细胞衰老,改善了卵子的生产. 这种细胞因子通过调节新陈代谢和关键途径来缓解衰老,为增强家禽养殖提供了潜在的潜力.

科学领域:

生殖生物学生殖生物学
细胞衰老细胞衰老
禽畜科学是一门学科.

背景情况:

繁殖期间的氧化应激会导致的粒粉细胞 (GC) 衰老,从而对蛋产量产生负面影响.
阿迪波涅丁 (ADPN) 是一种调节新陈代谢和抗氧化剂的细胞因子,在的GC衰老中没有明确的作用.

研究的目的:

调查ADPN在粒细胞 (GCs) 中调节氧化应激和衰老中的作用.
探索ADPN在缓解GC衰老和改善子产卵性能方面的潜力.

主要方法:

使用D-银糖诱导GC衰老,并通过RT-qPCR,SA-β-gal染色,免疫光,流细胞计和转录学进行评估.
操纵ADPN表达 (过度表达和淘汰) 以评估其对GC衰老的影响.
RNA测序 (RNA-seq) 用于识别由ADPN调节的分子通路.

主要成果:

在GC中,ADPN减轻了D-银糖诱导的氧化应激,细胞循环停止和SA-β-gal积累.
ADPN降低了与衰老相关的分泌表现型 (SASP) 基因 (IL-6,IL-8) 的表达,并保持了端粒长度.
通过AGE-RAGE和NOD类受体通路,ADPN调节了GC代谢能力和减弱衰老.

结论:

皮菌素 (ADPN) 有效地减轻了粒细胞 (GCs) 中的氧化应激和衰老.
ADPN对GC代谢和关键信号通路的调节作用提供了一种新的方法来提高的卵产量和寿命.

关键词:

亚迪波尼克丁氨酸是什么细胞衰老细胞衰老子子子子细胞质的花素.氧化应激是一种氧化应激.

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