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Watershed Planning within a Quantitative Scenario Analysis Framework
Published on: July 24, 2016
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废水样本存储用于物理化学和微生物学分析.
Gordon Webster1, Shrinivas Nivrutti Dighe2, William B Perry1
1School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Cardiff, Wales CF10 3AX, UK.
Journal of virological methods
|November 15, 2024
概括
基于废水的流行病学 (WBE) 样本储存影响标记物的完整性. 在4°C或-80°C储存样本对大多数标记物产生了类似的结果,这表明超低温度可能并不总是必要的,节省能源.
科学领域:
- 环境科学环境科学
- 公共卫生 公共卫生
- 微生物学 微生物学
背景情况:
- 基于废水的流行病学 (WBE) 对实时公共卫生监测至关重要.
- 样本完整性对于准确的WBE数据至关重要,从收集到分析.
- 优化样品储存条件对于可靠的WBE监控至关重要.
研究的目的:
- 评估不同冷藏条件对废水样本完整性的影响.
- 评估微生物和物理化学标记在各种储存温度和冷-解周期下的稳定性.
- 确定WBE的最佳存储协议,以平衡数据准确性和资源效率.
主要方法:
- 废水复合样本被储存在4°C,-20°C和-80°C的温度下.
- 在一年的时间内,样品经历了多达六次结解周期.
- 分析了微生物标记物 (核酸,SARS-CoV-2,crAssphage) 和物理化学参数.
主要成果:
- 储存温度显著影响了某些WBE标记物的保存.
- 对一些标记物的最高产量是在储存在-80°C的样本中观察到的.
- 大多数WBE标记在储存在-80°C和4°C之间没有显著的完整性差异.
结论:
- 最佳的储存条件对于保持废水样本对WBE的完整性至关重要.
- 在4°C下存档废水样本可能足以用于许多WBE标记,从而减少能源消耗和CO2排放.
- 这些发现支持开发具有成本效益和可持续的WBE存储协议,而不会影响数据可靠性.
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