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小膜蛋白CcoS参与了对cbb₃类型细胞染色体c氧化酶的辅因子插入

Juna Rauch¹, Katharina Kurscheidt¹, Kai-Wei Shen²

¹Institut für Biochemie und Molekularbiologie, ZBMZ, Faculty of Medicine, Albert-Ludwigs-Universität Freiburg, Freiburg, Germany.

Biochimica et biophysica acta. Bioenergetics

|November 15, 2024

概括

辅助蛋白CcoS对于 Rhodobacter capsulatus中的活性细胞染色体c氧化酶 (cbb3-Cox) 的组装至关重要. 它促进了亚单元的关联和和铜辅因子的插入.

科学领域:

生物化学生物化学
分子生物学分子生物学
微生物学微生物学

背景情况:

细胞染色体氧化酶是能量代谢中必不可少的多子单元蛋白质复合体.
这些复合体的组装需要辅助蛋白来进行子单元协调和辅助因子的传递.
在Rhodobacter capsulatus中,cbb3型细胞氧化酶 (cbb3-Cox) 的重要组合因子CcoS的功能以前是未知的.

研究的目的:

阐明CcoS蛋白在cbb3-Cox.组件中的功能.
调查CcoS在cbb3-Cox生物发生过程中的子单元关联和辅因子插入中的作用.

主要方法:

对缺乏CcoS (ΔccoS) 的Rhodobacter capsulatus菌株的分析.
化学交叉链接用于识别蛋白质与蛋白质相互作用.
调查组装复合体中的海姆和铜含量.

主要成果:

这种 ΔccoS 菌株产生了缺少血红素 b 的 cbb3-Cox,并导致 CcoN-CcoO 子单元的关联功能受损.
已经证明,CcoS与CcoN和CcoP子单元相互作用.
CcoS稳定了铜伴侣SenC和cbb3-Cox.之间的相互作用.
有证据表明,CcoS协调和铜的插入,而不是直接结合它们.

结论:

CcoS是cbb3-Cox的关键组装因子,对于适当的子单元协会至关重要.
与SenC一起,CcoS在协调和铜共因子插入cbb3-Cox.中发挥着关键作用.
这项研究揭示了在呼吸系统酶中辅因子递送和复杂组合的新机制.

关键词:

插入辅助因素的插入.这就是为什么Heme b b 罗多巴克特囊菌 (Rhodobacter capsulatus) 是一种细菌美国参议院/学校小膜蛋白,呼吸系统中的小膜蛋白.cbb(3) 类型的细胞染色体c氧化酶.

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