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纳米颗粒的抗菌作用装载纳米银和赫西丁在感染Enterococcus faecalis的根管中

Yiyi Wang¹, Linxin Fang², Peiling Wang¹

¹Stomatology Center, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China; The Academy of Medical Sciences, Zhengzhou University, Zhengzhou, China.

Journal of endodontics

|November 15, 2024

概括

携带纳米银和赫西丁 (nMS-nAg-Chx) 的半孔纳米颗粒有效抑制Enterococcus faecalis (E. faecalis) 和其生物膜. 这种新型化合物显示出作为内内治疗内牙治疗的有前途药物.

科学领域:

生物材料科学生物材料科学
微生物学微生物学
牙周内科医院牙周内科医院牙周内科医院

背景情况:

持续的微生物感染,特别是Enterococcus faecalis (E. faecalis),是导致内牙治疗失败的主要原因.
E. faecalis 是一个关键的病原体,与根管感染的持续性有关.

研究的目的:

调查带有纳米银和赫西丁 (nMS-nAg-Chx) 的中孔纳米颗粒对E. faecalis的抗微生物和抗生物膜作用.
评估nMS-nAg-Chx作为内治疗内治疗药物的潜力.

主要方法:

使用抑制区,最小抑制度 (MIC) 和生长曲线分析评估了对浮游生物E. faecalis的抗菌疗效.
通过测量乳酸,扫描电子显微镜 (SEM),活死染色和定量实时PCR (qRT-PCR) 来评估抗生物膜活性.
在感染的根通道中,使用殖民地形成单位 (CFU) 计数和在7天后用nMS-nAg-Chx.Chx封闭后的SEM来确定有效性.

主要成果:

nMS-nAg-Chx显示显著抑制浮游生物E. faecalis,其MIC为25μg/mL.
观察到E. faecalis生物膜形成的度依赖抑制,以减少乳酸,稀疏的生物膜结构,减少活力细菌和抑制毒性基因表达 (ebpR,gelE,ace,efa) 为证据.
与盐水对照相比,在使用nMS-nAg-Chx封闭7天后,感染根管内的细菌数量显著减少.

结论:

nMS-nAg-Chx对E. faecalis具有强大的抗微生物和抗生物膜活性.
该化合物有效地从感染的根管中消除了E. faecalis及其生物膜.
在内治疗中,nMS-nAg-Chx具有作为控制E. faecalis的内药物的显著潜力.

关键词:

菌 (Enterococcus faecalis) 是一种菌.半孔的二氧化纳米粒子.氧化的含量是多少牙周内牙周周周病牙周内牙周周病内治疗药物内药物纳米银的使用方法

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