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用一种新型等离子体pK432-IMP产生IMP-8的Enterobacter hormaechei的基因组分析

Jiao Feng¹, Zhiwei Song², Piaopiao Dai²

¹Institutes of Biomedical Sciences, Shanxi Provincial Key Laboratory of Medical Molecular Cell Biology, Shanxi University, Taiyuan, China.

Journal of global antimicrobial resistance

|November 15, 2024

概括

在中国发现了一种产生IMP-8的新型Enterobacter hormaechei (E. hormaechei) 菌株K432. 这项研究详细介绍了其基因组特征,并突出了一个新的等离子体,pK432-IMP,对于碳烯耐药性传播至关重要.

科学领域:

基因组学就是基因组学.
微生物学微生物学
传染性疾病传染性疾病

背景情况:

耐卡巴尼姆 Enterobacter cloacae (CR-ECC) 复杂感染在临床治疗方面存在重大挑战.
肠杆菌hormaechei (E. hormaechei) 是CR-ECC综合体中的一个主要的医院病原体.

研究的目的:

为了对多药耐药的E. hormaechei菌株K432.2进行全面的基因组表征.
确定有助于E. hormaechei.耐卡巴耐药性的新型遗传元素.

主要方法:

使用免疫结金技术检测卡巴基酶.
通过VITEK确定最小抑制度 (MIC) 值2.
对E. hormaechei K432菌株进行全基因组测序和分析.

主要成果:

从中国医院患者的尿液中分离出产生IMP-8的E. hormaechei菌株K432.
菌株K432表现出对多种抗生素的耐药性,包括卡巴烯和诺.
基因组分析揭示了一个染色体和三个等离子体 (pK432-IMP,pK432-TEM,pK432-NR) 携带六个抗性基因,包括bla-IMP-8在一个新型不相容组等离子体上.

结论:

这项研究提供了对E. hormaechei K432的详细基因组理解.
一种新的等离子体,pK432-IMP,被确定为IMP碳烯酶传播的关键载体.
建议对pK432-IMP型等离子体进行进一步监测,并对中国的多药耐药E. hormaechei进行例行监测.

关键词:

肠杆菌 hormaecheiii 的一个例子.bla{(IMP-8) 的使用情况.pK432-IMP 的使用情况

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Antibiotic Selection

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Plasmids are extrachromosomal DNA molecules found in bacteria, archaea, and some eukaryotic microbes like yeast. These small, circular DNA structures typically contain fewer than 30 genes, although some may exist linearly. Plasmids vary in their number within a cell, known as copy number. Single-copy plasmids are present in one copy per cell and multi-copy plasmids are present in multiple copies, reaching over 100 copies per cell.Plasmids usually replicate independently of the chromosomal DNA...