为辅助研究准备细胞学样本的工具,技术和挑战:ASC优化细胞学和小活检样本处理辅助研究工作组调查结果
Jonas J Heymann1, Cristiana M Pineda2, Christine N Booth3
1Department of Pathology and Laboratory Medicine, New York-Presbyterian Hospital-Weill Cornell Medicine, New York, New York.
Journal of the American Society of Cytopathology
|November 15, 2024
概括
细胞病理学辅助测试显示了实验室实践中的广泛差异. 样本采集协议的标准化是必要的,以提高诊断效用,并最大限度地减少预分析变化.
科学领域:
- 细胞病理学 细胞病理学
- 辅助测试 辅助测试 辅助测试
- 生物病理学 生物病理学
背景情况:
- 对细胞病理学和小活检样本的辅助测试对于诊断和临床决策至关重要.
- 测试结果受到预分析因素的显著影响,突出了对标准化样本采集协议的需求.
- 评估当前的机构实践对于确定需要在细胞病理学实验室中基于证据的标准的领域至关重要.
研究的目的:
- 调查关于辅助测试的细胞病理学实验室当前的做法.
- 为了识别样本采集和处理的变化.
- 确定限制辅助测试临床实用性的因素.
主要方法:
- 在2024年1月8日至3月1日期间,向美国细胞病理学会 (ASC) 会员分发了一份电子调查.
- 该调查包括12个问题,评估与辅助测试相关的实验室实践.
- 为了分析当前的机构实践,对答案进行了表化.
主要成果:
- 固定在形式素,嵌入式胺细胞块 (CBs) 广泛用于免疫组织化学 (89%),分子测试 (84%) 和现场杂交 (71%).
- 对样本采集媒介的做法各不相同,大多数人不使用任何媒介进行细针吸收,而液体基防腐剂对于皮标本是常见的.
- 低细胞性 (49%) 和缺乏验证 (23%) 被确定为主要因素限制辅助测试的临床效用.
结论:
- 目前的细胞病理学实验室实践存在显著差异,表明缺乏共识.
- 虽然细胞块的利用与先前的研究结果是一致的,但采集媒介的做法在样本类型之间有所不同.
- 该ASC已经建立了一个工作组,以促进标本标准化和减少预分析变化.
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