小型黄鱼 (Plagiognathops microlepis) 的染色体级基因组组合
Yangyang Liang1, Huijuan Liu2,3, Wenxuan Lu1
1Key Laboratory of Freshwater Aquaculture and Enhancement of Anhui Province, Fisheries Research Institute, Anhui Academy of Agricultural Sciences, Hefei, 230001, Anhui, China.
Scientific data
|November 15, 2024
概括
使用先进的测序,创建了一个小规模黄 (Plagiognathops microlepis) 的高质量基因组组. 这一资源将促进这一有价值的鱼类物种的遗传研究和水产养殖育种.
科学领域:
- 水产养殖的基因组学
- 进行比较的基因组学.
- 鱼类遗传学 鱼类遗传学
背景情况:
- 小型黄鱼 (Plagiognathops microlepis) 是东亚一个商业上重要的鱼类.
- 有限的基因组数据阻碍了P. microlepis.的遗传研究和繁殖.
研究的目的:
- 为了构建一个高保真性,染色体水平的基因组组合P. microlepis.
- 为P. microlepis.提供一个基础的基因组资源.
主要方法:
- 使用了PacBio Hifi长读测序和Hi-C技术.
- 组装了一个976.41 Mb的基因组,具有很高的连接性 (连接N50: 34.41 Mb,支架N50: 38.38 Mb).
- 使用BUSCO评分评估基因组完整性 (组装为98.08%,基因为96.98%).
主要成果:
- 在24个染色体中实现了99.84%的基因组分布.
- 确定了25389个编码蛋白质的基因,其中99.85%的功能注释.
- 对比合成分析揭示了与相关物种的染色体重排.
结论:
- 高质量的基因组组装是P. microlepis研究的关键资源.
- 能够在未来对基因组结构,功能和进化进行研究.
- 支持水产养殖繁殖和保护战略的进步.
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