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治疗性含有沙波宁的深层欧性溶剂,通过响应表面方法来优化,在体内对 Pseudomonas aeruginosa 产生强大的抗菌作用

Ramya M Subramani¹, Bhanuvalli R Shamprasad¹, Narayana Shri Sanjeev Viswanathan¹

¹Department of Chemistry, School of Chemical & Biotechnology, Shanmugha Arts, Science, Technology & Research Academy (SASTRA) Deemed University, Thanjavur, 613 401, Tamil Nadu, India.

Scientific reports

|November 15, 2024

概括

基于Quillaja Saponin和酸的设计型治疗性深度浸泡溶剂 (THEDES) 显示出对P. aeruginosa生物膜的强有力的抗菌活性. 这些新型的SMA-THEDES在体内有效,具有低毒性,表明它们作为抗菌生物材料的潜力.

科学领域:

生物材料科学生物材料科学
自然产品化学自然产品化学
抗菌研究抗菌研究

背景情况:

治疗性深层浸泡溶剂 (THEDES) 通过提高生物可用性,溶解性和透性来增强药物输送.
奎拉贾·萨尼 (Quillaja Saponin,简称SAP) 是一种三烯天然产品,为开发新型治疗剂提供了潜力.
抗微生物耐药性需要开发新的抗菌策略和生物材料.

研究的目的:

为了研究基于Quillaja Saponin (SAP) 的设计者THEDES的抗菌潜力.
评估基于SAP的THEDES对细菌生物膜,特别是P. aeruginosa的疗效.
使用响应表面方法 (RSM) 优化SAP-THEDES的组成,并评估其治疗潜力.

主要方法:

通过将SAP与各种α-酸 (酸,酸,酸,糖酸,乳酸) 和水相结合,合成了THEDES.
针对一组细菌菌株进行了抗菌活性和生物膜抑制试验.
使用Box-Behnken响应表面方法 (RSM) 来优化THEDES的组成.
使用斑马鱼感染模型评估了体内疗效,并进行了毒性研究.

主要成果:

沙宁-酸THEDES (SMA-THEDES) 在拆解P. aeruginosa生物膜方面表现出强大的活性.
优化的SMA-THEDES (SAP 1.014 m.mol,酸 1.003 m.mol,水 0.117 m.mol) 显示出对P. aeruginosa.的最大疗效.
在斑马鱼的体内研究表明,在SMA-THEDES治疗时,细菌殖民地数量下降了2倍以上.
毒性评估显示,肝脏和大脑酶水平或肝脏形态没有显著异常.

结论:

设计者SMA-THEDES是具有显著抗菌膜活性的有效治疗抗菌生物材料.
优化的SMA-THEDES在治疗P. aeruginosa感染方面表现有前途,毒性最小.
这项研究突出了以沙尼为基础的THEDES作为一种新型抗菌剂的潜力.

关键词:

伪omonas aeruginosa 这种类型的病毒.抗生物膜剂是一种抗生物膜剂.盒子-Behnken-响应表面的方法.治疗性深层感溶剂治疗性深层感溶剂

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