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相关概念视频

Proteomics01:33

Proteomics

7.2K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
7.2K

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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier
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审查和实用指南,以开始使用单细胞蛋白质组学.

Hsien-Jung L Lin1, Kei G I Webber1, Andikan J Nwosu1

  • 1Department of Chemistry & Biochemistry, Brigham Young University, Provo, Utah, USA.

Proteomics
|November 16, 2024
PubMed
概括

单细胞蛋白质组学 (SCP) 现在可以通过新的工具获得更多的实验室. 本指南为研究人员提供了实用步骤,以进行SCP实验并实现有意义的单细胞发现.

科学领域:

  • 蛋白质组学是指蛋白质组学.
  • 生物技术是生物技术.
  • 分析化学 分析化学

背景情况:

  • 单细胞蛋白组学 (SCP) 通过新的商业工具取得了重大进展.
  • 该领域现在已经成熟,可供实验室进行批量蛋白质组学.

研究的目的:

  • 为进入SCP领域的新研究人员提供实用指南.
  • 为了突出最近的工作,降低了SCP的进入障碍.

主要方法:

  • 概述了SCP实验的基本原则.
  • 详细说明样品的准备,分离,以及质谱数据的采集和分析.
  • 建议无标签的SCP工作流程,以获得简单,准确的结果.

主要成果:

  • 最近的进展使SCP更容易获得.
  • 建议使用无标签的工作流程,以方便使用和准确.
  • 研究人员可以自信地进行SCP实验.

结论:

  • 对于具有基本能力的研究人员来说,SCP领域是广泛可用的.
  • 实用的指导和可访问的方法使SCP的新研究人员有能力.
关键词:
细胞异质性的细胞异质性数据采集方法数据采集方法液体处理自动化的自动化样品的准备 样品的准备单细胞蛋白质组学 (单细胞蛋白质组学SCP)

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Droplet Barcoding-Based Single Cell Transcriptomics of Adult Mammalian Tissues
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  • 在单细胞水平上,SCP使得有意义的发现成为可能.