泽拉伦的酸化保持了它的毒性
Muhammad Asaduzzaman1, Ivan Pavlov1, Guillaume St-Jean2
1Département de Biomédecine vétérinaire, Faculté de Médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec J2S 2M2, Canada.
Journal of agricultural and food chemistry
|November 16, 2024
概括
微生物对泽拉伦 (ZEN) 的酸化产生了新产品 (ZEN-14-P,ZEN-16-P). 这些化形式保留了ZEN有害的细胞毒性,炎症和雌激素活性,表明排毒不足.
科学领域:
- 菌类毒理学 菌类毒理学
- 细胞生物学 细胞生物学
- 生物化学 生物化学
背景情况:
- 微生物生物转化正在探索如何使有害的真菌毒素zearalenone (ZEN) 失活.
- 目前尚不清楚新型ZEN酸化产品 (ZEN-14-P,ZEN-16-P) 的毒性和代谢命运.
研究的目的:
- 评估ZEN-14-P和ZEN-16-P的细胞毒性,氧化应激,炎症和雌激素作用.
- 为了确定生物系统中酸化ZENs的代谢命运和稳定性.
主要方法:
- 使用猪肠道 (IPEC-J2) 和人类子宫内膜 (石川) 细胞研究了细胞毒性.
- 评估了氧化应激,促炎性细胞因子表达和雌激素活性 (基因上调,性酸酶,腺增殖).
- 使用液体染色学-并联质谱法 (LC-MS/MS) 进行代谢命运分析.
主要成果:
- 化ZENs显著降低了IPEC-J2和石川细胞中的细胞活力.
- 化产品诱导了氧化应激,激活了前炎性细胞因子,并表现出类似于的雌激素活性.
- LC-MS/MS显示了化ZENs到ZEN的部分水解,具有不同的代谢途径.
结论:
- 微生物酸化可能不是为泽拉伦 (ZEN) 提供足够的排毒策略.
- 酸化ZEN产品保留了显著的细胞毒性,促炎性和雌激素性质.
- 需要进一步的研究来开发有效的ZEN排毒方法.
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