在一个实验室规模的反应堆中,微生物群落的进化运行以获得生物质,用于生物化学甲潜力测定
Victoria de la Sovera1, Patricia Bovio-Winkler1, Guillermo Zinola1
1Microbial Ecology Laboratory, Biological Research Institute "Clemente Estable" (IIBCE), Department of Biochemistry and Microbial Genomics, Av. Italia 3318, Montevideo, Uruguay.
Applied microbiology and biotechnology
|November 16, 2024
概括
一个实验室规模的生物反应器成功地为生物化学甲潜力 (BMP) 测试生产了可靠的甲原污泥. 在4°C下存储5个月的生物质并没有影响其活性或微生物组成,确保了一致的结果.
科学领域:
- 环境微生物学 环境微生物学
- 厌氧消化 厌氧消化
- 生物技术是生物技术.
背景情况:
- 生物化学甲潜力 (BMP) 测试对于评估废物的生物降解性和无氧消化中的毒性至关重要.
- 标准化和可存储的注射剂对于可重复的BMP测试结果至关重要.
- 乌拉圭当地一家企业利用实验室规模的生物反应器产生用于BMP测试的注射剂.
研究的目的:
- 评估用于注射生产的连续养生物反应器中的微生物群落的演变.
- 评估长期冷藏对储存生物质的甲生成活性和微生物组成的影响.
主要方法:
- 采用16S rRNA amplicon测序来分析细菌和考古社区的组成.
- 量化了甲原性活性,并使用mcrA定量PCR (qPCR) 来测量甲原体.
- 生物质在4°C存储了5个月,在储存前后进行了分析.
主要成果:
- 应用的方法产生了可靠的甲产物污泥,具有可接受的特定甲产量 (SMA).
- 微生物群体表现出对反应堆内不断增加和改变生物质的抵御力.
- 在4°C冷藏5个月并没有显著改变整体甲生成活性或微生物组成,尽管观察到mcrA丰度的变化.
结论:
- 开发的方法有效地生产适合BMP测试的生物质.
- 生物反应器内的微生物群体是强大的和适应性的.
- 适应气候生物质的长期冷藏储存是保持其适合BMP测试的可行策略.
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