线粒体病原性突变和COVID-19患者中氧化酸化基因的表达模式
Milad Shokuhi Nia1, Dormohammad Kordi Tamandani2, Mohammad Kazem Momeni3
1Department of Biology, University of Sistan and Baluchestan, Zahedan, Iran. milad_shokuhi@yahoo.com.
Iranian journal of allergy, asthma, and immunology
|November 16, 2024
概括
线粒体DNA突变和改变的氧化酸化基因表达与COVID-19的严重程度有关. 针对线粒体功能障碍为这种疾病提供了潜在的治疗策略.
科学领域:
- 遗传学 遗传学 是一个
- 分子生物学分子生物学
- 病毒学 病毒学
背景情况:
- 线粒体误解突变和病原性变体与COVID-19的病原性有关.
- 线粒体功能障碍越来越被认为是严重疾病结果的一个因素.
研究的目的:
- 评估线粒体DNA (mtDNA) 突变和基因表达变化在COVID-19进展中的作用.
- 为了将这些变化与临床特征和疾病严重程度相关联.
主要方法:
- 高通量下一代测序用于识别COVID-19患者和对照组的mtDNA突变.
- 生物信息工具用于预测突变对蛋白质结构和稳定性的影响.
- 定量实时聚合酶连锁反应来评估mtDNA编码的氧化酸化基因表达.
主要成果:
- 鉴定了八种不同的mtDNA突变,预计会降低蛋白质稳定性,包括CO3和ND5基因中独特的误解突变.
- 氧化酸化基因的下调与年龄的增加,白细胞,淋巴细胞,中性粒细胞和血小板数量的增加以及较高水平的肌素,ALT,AST和BUN相关.
- 这些分子变化与COVID-19疾病严重程度的增加有关.
结论:
- mtDNA突变和氧化酸化基因的改变表达有助于COVID-19中线粒体功能障碍.
- 线粒体功能障碍是COVID-19病变和严重性的关键因素.
- 准线粒体功能障碍为COVID-19治疗提供了一个有希望的治疗途径.
相关概念视频
Mitochondrial Membranes
8.9K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
8.9K
Electron Transport Chain: Complex I and II
11.9K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
11.9K
Animal Mitochondrial Genetics
7.5K
Among all the organelles in an animal cell, only mitochondria have their own independent genomes. Animal mitochondrial DNA is a double-stranded, closed-circular molecule with around 20,000 base pairs. Mitochondrial DNA is unique in that one of its two strands, the heavy, or H, -strand is guanine rich, whereas the complementary strand is cytosine rich and called the light, or L, -strand. Compared to nuclear DNA, mitochondrial DNA has a very low percentage of non-coding regions and is marked by...
7.5K
Mitochondrial Precursor Proteins
2.5K
Mitochondrial precursors are partially unfolded or loosely folded polypeptide chains. Newly synthesized precursors are inhibited from spontaneously folding into their native conformation by the cytosolic chaperones, heat shock proteins 70 (Hsp70), and mitochondrial import stimulation factors (MSFs). Precursors bound to MSFs are guided to the TOM70-TOM37 receptors, while precursors bound to Hsp70 chaperones are targetted to TOM20-TOM22 receptor complexes.
Most of the mitochondrial...
Most of the mitochondrial...
2.5K
Mitochondria
11.2K
Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
11.2K
Translocation of Proteins into the Mitochondria
3.0K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.0K


