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在Bacopa monnieri植物中使用Tamarindus indica的种子排泄物进行增强的化物合成

Titir Guha¹, Ekta Bhattacharya², Madhurima Dutta²

¹Agricultural& Ecological Research Unit, Biological Science Division Indian Statistical Institute, 203, Barrackpore Trunk Road, Kolkata, 700 108, India; Department of Biology, Indian Institute of Science Education and Research, Tirupati, Srinivasapuram, Yerpedu Mandal, Tirupati, 517619, Andhra Pradesh, India.

Plant physiology and biochemistry : PPB

|November 16, 2024

概括

塔马林 indica L. 种子排泄物 (TSE) 通过上调MVA通路中的关键基因来增强Bacopa monnieri中的化物产量. 这种可持续的方法提供了一种新的方法来提高这种重要的药用草本的产量.

科学领域:

植物生物化学和二次新陈代谢
基病变和植物间的相互作用.
药用草药的种植和增强药用草药的种植和增强

背景情况:

来自植物的各种类基因化学物质会影响目标物种,但通过种子排泄物相互作用的研究不足.
贝科巴·蒙尼耶里是一种有价值的药用草本,尽管药品需求很高,但其化物生物合成率很低.
使用外部压力因素提高植物的二次代谢物生产是一个活跃的研究领域.

研究的目的:

调查Tamarindus indica L.种子排泄物 (TSE) 在调节 Bacopa monnieri 的二次新陈代谢中的作用.
探索TSE作为一种可持续的方法来增强Bacopa monnieri中的化物生物合成.
为了确定TSE中负责调节Bacopa monnieri的新陈代谢的潜在基化学物质.

主要方法:

巴科帕蒙尼耶里种子被用不同度的TSE (1:16到1:2种子重量:水) 处理.
分析了二次代谢物含量,H2O2和酸水平.
进行了MVA通路 (BmAACT,BmHMGR,BmMDD,BmSQS,BmBAS) 的基因表达分析.
在LC-HRMS分析中,在TSE中确定了代基化学物质.

主要成果:

1:4的TSE度显著上调了二次代谢物含量.
通过TSE治疗增加了H2O2和酸的产生.
通过MVA途径参与化物生物合成的基因显示出上调.
与对照组相比,被TSE治疗的植物的化物水平高出1.7倍.
类化合物,和有机酸被确定为TSE的潜在活性化合物.

结论:

TSE有效调节Bacopa monnieri的二次新陈代谢,导致增强的化物生产.
该机制涉及增加的H2O2和酸,激活MVA通路.
已识别的等位基化学物质如卢巴宁,原酸和粘酸是关键的调节剂.
这项研究提出了一种可持续的策略,以增加植物中的化物产量.

关键词:

巴科帕·蒙尼耶里 (Bacopa Monnieri) 是一个化是化的一种.在MVA路径上.二次代谢产物二次代谢产物种子排泄物种子排泄物塔马林杜斯 (Tamarindus indica) 是一种表示性植物.

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