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使用向质谱仪检测食品中的沙门氏菌

Mengqi Chen¹, Miaoxi Peng², Muyun Yuan²

¹Guangzhou Customs Technology Center, Guangzhou 510623, China; School of Food Science and Engineering, South China University of Technology, Guangzhou 510641, China.

|November 16, 2024

概括

使用体生物标记物的新向性质谱法 (MS) 方法可以快速有效地检测食品中的Salmonella enterica. 这种方法与浮力密度离心相结合,显示出作为传统培养方法的替代方案的希望.

科学领域:

食品微生物学和分析化学.
开发用于食品安全的新型诊断技术.

背景情况:

沙门氏菌的高患病率需要改进检测方法.
针对性质谱 (MS),包括多重反应监测 (MRM) 和并行反应监测 (PRM),为病原体检测提供了更高的特异性和灵敏性.

研究的目的:

开发和验证一种新的向性MS方法,用于检测食品样本中的Salmonella enterica.
评估该方法区分沙门氏菌与其他食源性病原体的能力.
评估浮力密度离心法 (BDC) 的整合,用于样品制备.

主要方法:

开发一种针对性多发性硬化症的测定方法,使用一组由四种特定体生物标志物组成的小组来测定Salmonella enterica.
整合浮力密度离心法 (BDC) 来将沙门氏菌从复杂的食物矩阵中分离.
使用人工和自然污染的食品样本验证该方法,与标准培养方法进行比较.

主要成果:

这种新的向性多发性硬化症方法准确地区分了沙门氏菌从其他常见的食源性病原体中使用所选的生物标志物.
漂浮密度离心法 (BDC) 有效地将沙门氏菌从各种食品中分离出来.
基于MS的检测方法在真实食品样本中表现出与传统培养方法相比的性能.

结论:

开发的针对性MS方法与BDC相结合,提供了一种快速,具体和敏感的方法,用于在各种食品中检测沙门氏菌.
这种技术为食品安全测试的传统培养方法提供了可行的替代方案.
这些发现支持针对性MS在食品病原体监测中的更广泛应用.

关键词:

检测检测检测检测检测类生物标志物类生物标志物沙门氏菌 (Salmonella enterica) 已经成为一种常见的疾病.有针对性的质谱测量.

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