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相关概念视频

Colloidal precipitates01:09

Colloidal precipitates

517
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
517
One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation01:24

One-Compartment Open Model: Wagner-Nelson and Loo Riegelman Method for ka Estimation

398
This lesson introduces two critical methods in pharmacokinetics, the Wagner-Nelson and Loo-Riegelman methods, used for estimating the absorption rate constant (ka) for drugs administered via non-intravenous routes. The Wagner-Nelson method relates ka to the plasma concentration derived from the slope of a semilog percent unabsorbed time plot. However, it is limited to drugs with one-compartment kinetics and can be impacted by factors like gastrointestinal motility or enzymatic degradation.
On...
398
Analyte Adsorption and Distribution01:09

Analyte Adsorption and Distribution

608
In certain chromatographic separations, solutes transfer between the mobile phase and the stationary phase via sorption, which typically refers to the process of adsorption. For many chromatographic systems, the sorption process often depends on the polarity of the compounds—an expression of the overall dipole moment within the molecule. During the separation process, there is competition between the solute and solvent for adsorption to the stationary phase. Highly polar compounds and...
608
Precipitate Formation and Particle Size Control01:16

Precipitate Formation and Particle Size Control

737
In precipitation gravimetry, the precipitating agent should react specifically or selectively with the analyte. While a specific reagent reacts with the analyte alone, a selective reagent can react with a limited number of chemical species.
The obtained precipitate should be either a pure substance of known composition or easily converted to one by a simple process, such as ignition or drying. In addition, the precipitate should be insoluble and easily filterable. In general, filterability...
737
Colloids03:22

Colloids

17.4K
Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
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相关实验视频

Updated: Jun 7, 2025

Study of Short Peptide Adsorption on Solution Dispersed Inorganic Nanoparticles Using Depletion Method
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一种系统的方法来估计体颗粒吸附模型参数.

Oliver Lorenz-Cristea1, Angela Wiebe1, Judith Thoma1

  • 1DSP Development, Boehringer Ingelheim Pharma GmbH & Co. KG, Biberach, Germany.

Journal of chromatography. A
|November 16, 2024
PubMed
概括

一项新的战略系统地估计了体颗粒吸附 (CPA) 模型的参数,这对于生物制药下游工艺开发至关重要. 这种方法减少了准确的离子交换色谱建模所需的实验.

关键词:
体颗粒吸附模型的模型机械色谱模型机械色谱模型参数估计的参数估计.蛋白质净化 蛋白质的净化灵敏度分析是一种灵敏度分析.代理辅助的全球优化优化

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Monitoring Protein Adsorption with Solid-state Nanopores
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相关实验视频

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科学领域:

  • 生物化学工程 生物化学工程
  • 染色体学 染色体学 是一种染色学.
  • 过程开发 过程开发

背景情况:

  • 精确的离子交换色谱 (IEX) 模型参数对于生物制药下游工艺开发至关重要.
  • 目前的模型校准方法由于模型的限制和参数相关性而面临挑战,导致效率低下.
  • 在新兴的体粒子吸附 (CPA) 模型中,需要一种系统的方法来估计参数.

研究的目的:

  • 提出一个新的,系统的策略来估计CPA模型参数.
  • 提高模型辅助生物制药下游流程开发的效率和准确性.
  • 为了能够在各种负载条件和化模式中预测化行为.

主要方法:

  • 执行参数灵敏度分析以确定关键估计杆.
  • 使用突破性和梯度化实验开发了一个最小实验数据集策略.
  • 使用了代理辅助的全球优化工具和定制的目标函数,以实现高效的参数拟合.
  • 在使用强基离子交换染色学对三种模型蛋白 (50,150,200 kDa) 验证了该方法.

主要成果:

  • 确定了CPA模型估计的关键参数及其相关性结构.
  • 在梯度和阶段化中,成功预测了低和高负载密度的化行为.
  • 建立了一个最小的实验设计,只需要一个突破,一个高负荷和三个低负荷梯度化实验.
  • 实现单个组件的高通量CPA模型校准.

结论:

  • 开发的策略为CPA模型参数估计提供了显著的改进.
  • 经过验证的CPA模型准确地描述了各种实验条件,包括无约束脉冲,梯度/异位化和突破.
  • 这种方法提高了生物制药的模型辅助下游过程开发的效率.