从人类宿主中分离出来的Fasciolopsis buski,印度基于核核糖体DNA序列的独特类
Sandhya Chaurasiya1, Aradhana Singh1, Anurag Tiwari2
1Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi, Uttar Pradesh, India.
Indian journal of medical microbiology
|November 16, 2024
概括
由Fasciolopsis buski引起的Fasciolopsiasis是一个重大的健康问题. 遗传分析显示,印度Fasciolopsisbuski从人类和猪中分离出来,与越南和中国菌株有很密切的关系.
科学领域:
- 寄生虫学的寄生虫学
- 分子生物学分子生物学
- 公共卫生 公共卫生
背景情况:
- 由寄生虫虫Fasciolopsis buski引起的Fasciolopsiasis是人类发病的一个显著来源.
- 这项研究重点关注了在印度北方邦的一名患者中发现的Fasciolopsis buski感染.
研究的目的:
- 使用28S rDNA和ITS2遗传区域进行Fasciolopsis buski的遗传学分析.
- 研究来自不同地理位置和宿主的Fasciolopsis buski分离物之间的遗传关系.
主要方法:
- 从患者内镜检查中获得的Fasciolopsis buski标本中提取DNA.
- 用28S核糖体DNA (rDNA) 和内部转录间隔器2 (ITS2) 区域进行的遗传学分析.
主要成果:
- 印度Fasciolopsisbuski分离物与印度梅加拉雅州的猪原产物分离物具有很高的遗传相似性.
- 对于28S rDNA的物种内遗传变异在印度为1-3%,在越南为5%.
- 对ITS2的物种内遗传变异在印度为1-2%,在越南为17%.
结论:
- 在印度,从人类和猪中分离出来的Fasciolopsis buski表现出密切的遗传关系.
- 地理分离有助于遗传分歧,印度的分离物比越南和中国的分离物更相似,它们形成了不同的分类.
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