相关实验视频

Updated: Jun 7, 2025

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing

Published on: October 15, 2019

双向门德尔随机化将肠道微生物群与原发性胆道胆道炎联系起来

Zhijia Zhou^1,2, Wenxuan Li^1,2, Yuelan Wu^1,2

¹Department of Hepatology, ShuGuang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, 201203, China.

Scientific reports

|November 16, 2024

概括

一些肠道细菌,如Coriobacteriales,增加了原发性胆道胆炎 (PBC) 的风险,而其他细菌,如Deltaproteobacteria,则降低了风险. 调节肠道微生物群可能为PBC提供新的治疗策略.

科学领域:

胃肠病学胃肠病学
微生物学微生物学
遗传学是一个遗传学.

背景情况:

原发性胆道胆炎 (PBC) 和肠道微生物群 (GM) 具有共同的流行病学联系,但因果关系尚不清楚.
了解这些联系对于开发用于PBC的新型治疗干预措施至关重要.

研究的目的:

使用孟德尔随机化研究肠道微生物群和初级胆道胆炎之间的因果关系.
探索特定肠道细菌种群和PBC之间的双向因果关系.

主要方法:

利用肠道微生物群 (MiBioGen) 和PBC.的大规模GWAS数据.
采用双向双样本的孟德尔随机化 (MR) 与反变差加权,MR-Egger,加权中位数和MR-PRESSO方法.
通过使用科克兰的Q统计和MR-Egger拦截测试来评估异质性.

主要成果:

基因决定的Coriobacteriales序列与增加的PBC风险相关 (OR=2.18).
基因确定的Deltaproteobacteria类在降低NAFLD风险方面表现出因果关系 (OR=0.52).
PBC的进展与增加的细菌菌群,细菌菌群和细菌菌群的丰富性有关,并减少了Lachnospiraceae的丰富性UCG010.

结论:

基因驱动的肠道细菌,特别是Coriobacteriales序列和Deltaproteobacteria类,在PBC风险中起着因果作用.
PBC本身影响了特定肠道微生物群的丰富性,包括Bacteroidia, Bacteroidales, Bacteroidetes和Lachnospiraceae UCG010.
调节肠道微生物群为治疗初级胆道胆炎提供了潜在的治疗途径.

关键词:

这是双向的双向.因果关系是因果关系.我们的肠道微生物群.门德尔的随机化原发性胆道胆管炎是一种胆道胆管炎.

更多相关视频

Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method

Analysis of Fecal Microbiota Dynamics in Lupus-Prone Mice Using a Simple, Cost-Effective DNA Isolation Method

Published on: May 2, 2022

Chronic Salmonella Infection Induced Intestinal Fibrosis

Chronic Salmonella Infection Induced Intestinal Fibrosis

Published on: September 22, 2019

相关概念视频

Chronic Bowel Disorders: Introduction

Chronic Bowel Disorders: Introduction

Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...

Irritable Bowel Syndrome I: Introduction

Irritable Bowel Syndrome I: Introduction

Irritable Bowel Syndrome (IBS) is characterized by functional disturbances in the gastrointestinal system, presenting a cluster of symptoms without evident structural or biochemical abnormalities. It primarily affects the large intestine and may cause abdominal pain, bloating, excessive gas, diarrhea, constipation, or both.
IBS is a chronic condition that can persist over a long period or recur frequently.
The pathogenesis of IBS involves a complex interplay of the following factors:
Altered...

Pedigree Analysis

Pedigree Analysis

Anatomy of the Intestines

Anatomy of the Intestines

Although digestion of proteins, carbohydrates, and lipids may begin in the stomach, it is completed in the intestine. The absorption of nutrients, water, and electrolytes from food and drink also occurs in the intestine. The intestines can be divided into two structurally distinct organs—the small and large intestines.
Small Intestines
The small intestine is an ~7 meter-long tube with an inner diameter of just 2.5 cm. Since most nutrients are absorbed here, the inner lining of the...