代谢状态是影响母羊粒粉细胞蛋白质变化的关键因素,由慢性BPS暴露引起
Marie-Emilie Lebachelier de la Riviere1, Ophélie Téteau1, Coline Mahé1
1INRAE, CNRS, Université de Tours, PRC, Nouzilly, 37380, France.
BMC genomics
|November 16, 2024
概括
双S (BPS) 暴露会破坏母羊的卵巢功能,改变颗粒细胞蛋白质组. 代谢状态会影响BPS的影响,可能会延长其内分泌干扰,特别是在肥胖个体中.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 蛋白质组学是指蛋白质组学.
背景情况:
- 双S (BPS) 是BPA的常见替代品,BPA是一种已知的内分泌干扰物.
- 已知BPS暴露会破坏卵巢功能.
- 在粒粉细胞蛋白质上,BPS效应与代谢状态之间的相互作用仍然没有表征.
研究的目的:
- 为了研究慢性BPS暴露对母羊颗粒细胞蛋白质组的影响.
- 探索代谢状态和BPS诱导的生殖效应之间的相互作用.
主要方法:
- 40只母羊被分配给受限制的 (R) 或养良好的 (WF) 饮食.
- 绵羊每天接受BPS剂量 (0或50μg/kg/天) 持续3个月.
- 用纳米-LC-MS/MS进行了花粉素细胞的蛋白质组分析.
主要成果:
- 根据代谢状态,慢性BPS暴露改变了颗粒细胞蛋白质组的不同程度.
- 确定了59种不同丰富的蛋白质,主要是在碳水化合物和脂质通路中.
- BPS上调的β-葡萄糖酶 (GUSB),一种酶,可解BPS葡萄糖化物 (BP-g),延长BPS的活性.
结论:
- 通过BPS对GUSB的升级,可以通过增加自由BPS水平来扩展其雌激素效应.
- BPS与代谢状况 (尤其是肥胖) 之间的相互作用可能导致长期和加重暴露.
- 这些发现凸显了BPS的有害影响及其与宿主新陈代谢的相互作用.
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