相关实验视频

Updated: Jun 7, 2025

Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue

Published on: February 13, 2019

血管活性肠 (VIP) 和其脂肪组织中的受体:对寒冷应激适应的影响

Orhan Tansel Korkmaz¹, Faruk Saydam², Bahar Dalkiran³

¹Department of Physiology, Faculty of Medicine, Eskisehir Osmangazi University, 26040, Eskisehir, Turkey. otkorkmaz@ogu.edu.tr.

Cell biochemistry and biophysics

|November 17, 2024

概括

血管活性肠 (VIP) 和它的受体存在于白色和棕色脂肪组织中. 暴露于寒冷会增加VIP及其受体水平,这表明在寒冷压力期间在代谢适应中发挥了作用.

科学领域:

生理学生理学生理学
内分泌学在内分泌学.
代谢过程中的代谢.

背景情况:

脂肪组织是生存至关重要的动态器官,参与免疫,哺乳,新陈代谢和热生成.
最近的研究强调脂肪组织的复杂组织,可塑性和神经血管供应.
血管活性肠 (VIP) 和其受体 (VPAC1,VPAC2) 参与各种生理过程,但它们在脂肪组织中的作用尚未得到研究.

研究的目的:

在老鼠白色脂肪组织 (WAT) 和棕色脂肪组织 (BAT) 中研究VIP及其受体 (VPAC1,VPAC2) 的存在和活性.
确定寒冷压力对VIP及其受体表达及其脂肪细胞和脂肪组织中的蛋白质水平的影响.

主要方法:

分析了从正常和冷压鼠的 inguinal WAT 和 interscapular BAT 中分离的脂肪细胞和脂肪组织.
评估了VIP,VPAC1和VPAC2的基因表达.
VIP,VPAC1和VPAC2的蛋白质水平使用西方斑点分析来确定.

主要成果:

在正常条件下,在WAT和BAT中检测到VIP和VPAC1基因表达;VPAC2缺席.
寒冷暴露在WAT和BAT中对VIP基因表达进行了上调.
寒冷压力诱导了VPAC2基因表达,而VPAC1在BAT中没有显著变化,而WAT略有下降. 在暴露于寒冷后,VIP,VPAC1和VPAC2的蛋白质水平在WAT和BAT中显著增加.

结论:

在白色和棕色脂肪组织中,VIP及其受体 (VPAC1,VPAC2) 存在并由冷应激调节.
脂肪组织中的VIP信号在适应寒冷压力的过程中起作用,影响新陈代谢和生化功能.
这些发现表明VIP作为代谢障碍的治疗点的潜力.

关键词:

脂肪组织的脂肪组织.寒冷的压力是冷的压力.热生成是一种热生成.在VPAC1中使用.在VPAC2中使用.血管活性肠道 (VIP)

更多相关视频

Author Spotlight: The Significance of Isolation, Culture, and Adipogenic Induction of SVF-Derived Preadipocytes from Mouse Perivascular Adipose Tissue

Author Spotlight: The Significance of Isolation, Culture, and Adipogenic Induction of SVF-Derived Preadipocytes from Mouse Perivascular Adipose Tissue

Published on: July 21, 2023

Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads

Measurement of Basal and Forskolin-stimulated Lipolysis in Inguinal Adipose Fat Pads

Published on: July 21, 2017

相关概念视频

Responses to Heat and Cold Stress

Responses to Heat and Cold Stress

Every organism has an optimum temperature range within which healthy growth and physiological functioning can occur. At the ends of this range, there will be a minimum and maximum temperature that interrupt biological processes.

Regulation of Food Intake

Regulation of Food Intake

Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...