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暴露于聚乙烯纳米塑料会通过线粒体ROS的产生和功能障碍促进细胞过早衰老,在预分化的骨肌细胞质中出现功能障碍

EunJin Bang¹, Hyun Hwangbo¹, Hyesook Lee²

¹Basic Research Laboratory for the Regulation of Microplastic-Mediated Diseases, Dong-eui University, Busan 47227, Republic of Korea; Department of Biochemistry, College of Korean Medicine, Dong-eui University, Busan 47227, Republic of Korea.

|November 17, 2024

概括

聚乙烯纳米塑料 (PS-NPs) 通过破坏线粒体,在衰老的肌肉细胞中引起细胞衰老. 这项研究揭示了PS-NPs诱导线粒体功能障碍,导致肌肉细胞衰老和潜在的健康风险.

科学领域:

环境健康环境健康
细胞生物学细胞生物学
毒理学毒理学毒理学

背景情况:

纳米塑料 (NP) 是普遍存在的环境污染物,具有潜在的人类健康风险.
骨肌肉衰老的特点是肌肉发育的恶化和肌肉质量损失.
在衰老的肌肉细胞中,聚乙烯纳米塑料 (PS-NP) 毒性的特定机制尚不清楚.

研究的目的:

为了研究PS-NP在预分化的C2C12髓细胞中的细胞内化,聚合和衰老效应.
为了阐明线粒体功能障碍在PS-NP诱导的细胞衰老中的作用.
评估PS-NP对髓细胞增殖和衰老的影响.

主要方法:

预先分化的C2C12细胞核细胞暴露在PS-NP的不同度下.
流细胞计和传输电子显微镜 (TEM) 用于NP内部化分析.
评估衰老标志物 (β-银酸酶,p16,p21),细胞周期,线粒体功能 (超氧化物产生,ATP水平) 和细胞增殖.

主要成果:

PS-NP被肌细胞内化,导致衰老特征增加和细胞循环停止.
暴露于PS-NP诱导了显著的线粒体损伤,包括超氧化物产生,膜脱极化和减少ATP合成.
抑制线粒体功能加剧了PS-NP毒性,而清除线粒体超氧化物减轻了衰老并恢复了增殖.

结论:

通过线粒体超氧化物生产和功能障碍,PS-NPs通过线粒体超氧化物生产和功能障碍诱导先前分化的肌细胞中的细胞衰老.
线粒体健康是肌肉细胞中PS-NP毒性的关键目标.
这些发现凸显了纳米塑料对骨肌肉健康的潜在风险,特别是在老年人群中.

关键词:

细胞衰老细胞衰老线粒体功能障碍线粒体功能障碍线粒体的超氧化物.脑细胞质细胞脑细胞质细胞是什么?聚烯纳米塑料的聚烯纳米塑料.

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