不同的提取温度对 dandelion 叶中的多糖的结构特征和抗氧化活性的影响
Pei Chen1, Ee Sang1, Huanhuan Chen1
1School of Pharmacy & School of Biological and Food Engineering, Changzhou University, Changzhou 213164, PR China..
International journal of biological macromolecules
|November 17, 2024
概括
低温提取产生具有卓越抗氧化特性的 dandelion 叶多糖化物 (DLP). 这些发现表明,4°C提取的DLP是食品,药物和生物材料应用的理想选择.
科学领域:
- 植物生物化学 植物生物化学
- 食品科学 食品科学 食品科学
- 生物材料科学是生物材料的科学.
背景情况:
- 花多糖 (DLP) 具有多样化的生物活性.
- 提取温度显著影响多糖的特性和有效性.
- 了解这些影响对于优化DLP应用至关重要.
研究的目的:
- 调查提取温度 (4°C与80°C) 对 dandelion叶多糖化合物 (DLP) 结构和抗氧化活性的影响.
- 为了比较在不同的热条件下获得的DLP的抗氧化潜力.
主要方法:
- 在4°C和80°C中提取DLP.
- 使用离子染色学 (IC),FT-IR和甲基化技术进行分析.
- 通过DPPH,ABTS,OH激素清除,降低功率测试和活性氧物种 (ROS) 测试来评估抗氧化活性.
- 使用超氧化解酶 (SOD) 和甲 (MDA) 测量对体外氧化损伤保护的评估.
主要成果:
- 更高的提取温度增加了产量,但分子重量下降.
- DLP4和DLP80都是复杂的异多聚糖,主要由银河糖,阿拉比诺糖,葡萄糖和曼诺糖组成.
- 与DLP80.0相比,低温提取的DLP4具有更高的6-银糖含量,并表现出更强的抗氧化活性和对氧化应激的保护作用.
- 在两种DLP类型中,热处理诱导了形状变化.
结论:
- 低温提取 (4°C) 产生的DLP具有增强的结构完整性和优越的抗氧化能力.
- 在较低温度下提取的DLP在食品,药物和生物材料中显示出更大的天然抗氧化剂潜力.
- 优化提取温度是最大限度地发挥 dandelion 叶多糖的功能益处的关键.
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