激活NR1D1通过抑制牛子宫内膜上皮细胞IL-6表达来缓解炎症反应
Wanghao Yang1, Mengdong Jin1, Yiqun Wang1
1Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China; Key Laboratory of Animal Biotechnology of the Ministry of Agriculture and Rural Affairs, Northwest A&F University, Yangling 712100, Shaanxi, China.
International journal of biological macromolecules
|November 17, 2024
概括
核受体子家族1组D成员1 (NR1D1) 的激活减少了奶牛子宫内膜炎的炎症. 针对NR1D1提供了一种新的治疗策略,以对抗这种昂贵的乳制品行业疾病.
科学领域:
- 兽医医学 兽医医学 兽医医学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 牛子宫内膜炎在乳制品行业造成重大经济损失.
- 目前的抗生素治疗面临的挑战是细菌耐药性和牛奶残留物.
- 有效的子宫内膜炎管理需要新的治疗目标.
研究的目的:
- 调查核受体子家族1组D成员1 (NR1D1) 在牛子宫内膜炎中的作用.
- 阐明NR1D1影响子宫内膜细胞炎症反应的潜在机制.
- 评估NR1D1作为乳牛子宫内膜炎的潜在治疗标.
主要方法:
- 使用牛子宫内膜上皮细胞系 (BENDs) 和用大肠杆菌脂多糖化物 (LPS) 诱导的初级细胞.
- 通过免疫光和免疫组织化学评估NR1D1表达.
- 研究了NR1D1对细胞因子表达 (IL-6,IL-1β,IL-8,CCL5) 的影响,使用基因删除,过度表达和激动剂 (SR9009) 治疗.
- 雇佣的双化酶记者测试分析NR1D1与IL-6促进体的相互作用.
主要成果:
- 在BEND中,LPS显著增加了NR1D1和促炎性细胞因子表达.
- 在患有子宫内膜炎的奶牛的子宫组织中观察到NR1D1表达的升高.
- 通过激素SR9009的NR1D1激活,减弱了LPS诱导的炎症性细胞因子表达在体外和体内 (小鼠).
- NR1D1抑制了IL-6促进体活性,表明转录抑制是关键机制.
结论:
- 激活NR1D1通过抑制促炎性细胞因子表达,特别是IL-6,缓解牛子宫内膜细胞的炎症反应.
- NR1D1至少部分通过特定的响应元件通过IL-6促进体起作用.
- NR1D1代表了预防和治疗乳牛子宫内膜炎的有前途的治疗标.
关键词:
循环节的时钟是循环节的时间.乳牛牛奶牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛牛在子宫内膜炎中,子宫内膜炎.介乐金-6 介乐金-6 是一种这就是NR1D1的特点.在子宫,子宫.更多相关视频
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