[非典型的非Typhi 沙门氏菌肠道细菌病,乳糖阳性,氨酸脱碳酶阴性]
Susana D García1, María I Caffer2, Marisa N Almuzara1
1Departamento de Bioquímica, Clínica. Hospital de Clínicas «Gral. José de San Martín», Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina; Instituto de Fisiopatología y Bioquímica Clínica (INFIBIOC), Cátedra de Microbiología Clínica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Buenos Aires, Argentina.
Revista Argentina de microbiologia
|November 17, 2024
概括
这项研究报告了阿根廷首次从患有系统性红性狼的患者中分离出一种非典型的非Typhi Salmonella enterica菌株. 质谱测量对于准确的鉴定至关重要,突出了标准生物化学测试可能导致的误识.
科学领域:
- 临床微生物学 临床微生物学
- 传染病 传染病 传染病
- 细菌学 细菌学是一门学科.
背景情况:
- 非Typhi Salmonella enterica (NTSE) 感染可以导致严重的全身疾病.
- 准确识别细菌病原体对于有效治疗至关重要.
- 沙门氏菌的非典型表现可能会挑战常规的诊断方法.
研究的目的:
- 报告在阿根廷首次分离出一种乳糖发酵,氨酸脱碳酶阴性NTSE.
- 评估质谱与生物化学测试的性能,以识别非典型的沙门氏菌菌株.
- 为了提高人们对阿根廷这种特定的沙门氏菌菌株的传播的认识.
主要方法:
- 来自患有系统性红斑狼的患者的血液培养.
- 使用矩阵辅助激光脱/离子化飞行时间 (MALDI-TOF) 质谱法进行识别.
- 与VITEK® 2系统和传统的生物化学试验进行比较.
- 对体质抗原OMA进行血清检测.
- 在国家参考中心确认.
主要成果:
- 隔离一种乳糖发酵,氨酸脱碳酶阴性NTSE菌株.
- 质谱学确定了S. enterica (得分: 2.148).
- 维特克® 2系统将其识别为"沙门氏菌群" (86%的歧视).
- 生物化学测试表明Citrobacter freundii复合体. 这是一个很好的例子.
- 血清检测结果对OMA呈阳性.
- 已确认为沙门氏菌亚种. 我 4,[5],12:i:-不典型的.
结论:
- 质谱法对于识别非典型的微生物分离物非常有价值,克服了生物化学测试的局限性.
- 这种非典型的沙门氏菌菌株正在阿根廷传播,这可能是一个潜在的诊断挑战.
- 临床实验室应该了解这种菌株,以便准确诊断和患者管理.
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