相关实验视频

Updated: Aug 28, 2026

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

长时间的培养增强了hiPSC中细胞前代衍生条件介质的刺激活性

Darja Marolt Presen^1,2, Vanessa Goeschl^3,4, Dominik Hanetseder^3,4

¹Ludwig Boltzmann Institute for Traumatology, The Research Centre in Cooperation with AUVA, Donaueschingenstrasse 13, Vienna, A-1200, Austria. darja.marolt.presen@ki.si.

Stem cell research & therapy

|November 17, 2024

概括

来自人类诱导的多能干细胞衍生的间酶原体 (hiPSC-MP-CM) 的条件介质增强了成年/老年捐赠者的早期骨细胞分化. 长期种植hiPSC-MPs可以促进CM.

科学领域:

干细胞生物学干细胞生物学
再生医学是一种再生医学.
组织工程是组织工程.

背景情况:

人类诱导的多能干细胞 (hiPSCs) 为再生疗法提供了一个可扩展的来源.
从hiPSCs (hiPSC-MPs) 衍生出的介质细胞原体可以产生用于治疗的秘密体.
调查hiPSC-MP条件介质 (CM) 刺激骨细胞分化是至关重要的.

研究的目的:

评估hiPSC-MP-CM在促进人类骨髓衍生中酶体 stromal 细胞 (MSCs) 的骨质基因分化方面的潜力.
确定hiPSC-MPs的长时间培养或预分化是否会增强其CM的骨质性刺激活性.
为了识别hiPSC-MP-CM中的分子组件,负责其治疗效果.

主要方法:

从广泛的年龄范围 (20-90岁) 的13位捐赠者中分离出了多重质细胞.
用hiPSC-MP-CM处理了MSC培养物,并在14天和6周评估了增殖和骨质分化.
对在不同条件下培养的hiPSC-MPs的CM进行了比较蛋白质组分析.

主要成果:

hiPSC-MP-CM显著增加了成年/老年捐赠者的MSC中的性酸酶的扩散和活性.
在6周后,CM补充剂没有改善MSC颗粒矿化.
长期培养 (14天) 的hiPSC-MPs,但没有骨质原性预分化,增强了CM的刺激活性.
蛋白质组分析揭示了增强的CM中独特的蛋白质配置文件,与细胞骨组织和细胞外矩阵组件有关.

结论:

hiPSC-MP-CM有效地增强了人类骨髓衍生的MSCs早期骨质性分化.
长期种植hiPSC-MPs是扩大其CM的骨质生成潜力的关键.
蛋白质组的洞察力为优化hiPSC-MP-CM为骨再生应用铺平了道路.

关键词:

衰老的衰老衰老的衰老有条件的介质.人类骨髓中介质层细胞是骨髓中介质层细胞.人类的 iPSCs.介质细胞的祖先.骨质变异的分化骨质变异的分化蛋白质组分析蛋白质组分析一个秘密,一个秘密.

更多相关视频

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Published on: July 18, 2017

Author Spotlight: Advancements in iPSCs and Genetic Disease Research

Author Spotlight: Advancements in iPSCs and Genetic Disease Research

Published on: October 20, 2023

相关概念视频

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.