相关实验视频

Updated: Jun 7, 2025

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Rapid Neuronal Differentiation of Induced Pluripotent Stem Cells for Measuring Network Activity on Micro-electrode Arrays

Published on: January 8, 2017

在人体诱导多能干干细胞中获得的神经网络上的体外电生理学药物测试

Giulia Parodi¹, Giorgia Zanini², Linda Collo²

¹Department of Informatics, Bioengineering, Robotics, and Systems Engineering (DIBRIS), University of Genova, Genova, Italy. giulia.parodi@edu.unige.it.

Stem cell research & therapy

|November 17, 2024

概括

人类诱导的多能干细胞衍生神经网络在暴露于离子受体对抗剂时显示出显著的电生理学变化. 这项研究描述了药物对这些药物测试模型中的谷氨酸和GABAergic传播的药物影响.

科学领域:

神经科学是一个神经科学.
干细胞生物学干细胞生物学
药理学药理学是指药理学的学科.

背景情况:

使用神经元培养和微电极阵列 (MEA) 的体外模型为药物测试提供了简化的体外样式试验.
虽然动物模型中的化学调制效应得到了充分的研究,但人类诱导多能干细胞 (hiPSC) 衍生神经网络中电生理学变化的特征缺乏.

研究的目的:

描述药理学操纵对不同配置的hiPSC衍生神经网络的电生理学影响.
为了研究电离体谷氨酸和GABA受体对抗剂对神经网络活动的影响.

主要方法:

建立了三种hiPSC衍生的神经网络配置:完全谷氨酸性 (100E),75%谷氨酸性/25%GABAergic (75E25I) 和完全GABAergic (100I).
适用于NMDA受体 (APV),AMPA受体 (CNQX) 和GABA_A受体 (比库库林,皮克罗托克辛,乙etrazole) 的对抗剂.

主要成果:

在所有测试的配置中,APV和CNQX取消了网络爆破并改变了功能连接.
GABA_A受体对抗剂 (BIC,PTX,PTZ) 增加了发射和爆发活动,并增强了功能连接,特别是在具有抑制成分的网络中.

结论:

由hiPSC衍生的神经网络对激发性和抑制性神经传递的药理学调制高度敏感.
这些发现代表了利用人类衍生的神经元网络用于先进的药物测试应用的关键一步.

关键词:

药物测试是指对药物进行测试.电子生理学电子生理学人类诱导的多能干细胞微电极阵列微电极阵列

更多相关视频

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Synaptic Microcircuit Modeling with 3D Cocultures of Astrocytes and Neurons from Human Pluripotent Stem Cells

Published on: August 16, 2018

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

Post-differentiation Replating of Human Pluripotent Stem Cell-derived Neurons for High-content Screening of Neuritogenesis and Synapse Maturation

Published on: August 28, 2019

相关概念视频

EPS and iPS Cells in Disease Research

EPS and iPS Cells in Disease Research

Embryonic and induced pluripotent stem cells are excellent models for disease research because of their ability to self-renew and differentiate into most cell types. Somatic cells from a patient are isolated and reprogrammed into induced pluripotent stem cells or iPSCs. These iPSCs are later differentiated into the desired cell type, which mirrors the diseased cell of the patient. In this way, disease models have been created for investigating diseases such as Down syndrome, type I diabetes,...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...