相关实验视频

Updated: May 3, 2026

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Basophil Activation Test for Investigation of IgE-Mediated Mechanisms in Drug Hypersensitivity

Published on: September 16, 2011

定义最佳的基细胞被动敏感化参数

Santiago Alvarez-Arango^1,2, Melanie C Dispenza¹, Kristin L Chichester¹

¹Division of Allergy and Clinical Immunology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology

|November 18, 2024

概括

优化基细胞激活测试可以改善药物特异性IgE检测以诊断过敏症. 这项研究为基细胞敏感化提供了指导方针,提高了药物过敏的诊断准确性.

科学领域:

免疫学免疫学免疫学
过敏诊断过敏诊断
细胞检测细胞检测

背景情况:

药物引起的过敏反应的准确诊断依赖于检测药物特异性IgE (sIgE).
基细胞激活试验 (BAT) 对于识别药物-sIgE至关重要,需要对分析进行优化.
优化BAT涉及了解影响基细胞对sIgE反应的因素.

研究的目的:

调查影响基细胞响应值的复杂因素.
建立基细胞敏感化试验中预期结果的基本规则.
通过基细胞激活来完善检测药物特异性IgE的方法.

主要方法:

分析了使用流细胞计对基细胞的FcεRI受体表达.
用D2O和/或IL-3进行化,具有不同度的青素 (BPO) -sIgE的敏感化基细胞.
根据FcεRI密度,sIgE/总IgE比率以及对刺激的反应来评估基因细胞敏感性.

主要成果:

在优化条件下 (4小时在4°C以IL-3和70%D2O进行敏感化) 检测出低BPO-sIgE/tIgE比率 (0.02%-0.05%).
最大抗IgE反应的平均值为58%,FcεRI受体表达率为~155,000/细胞.
在优化条件下检测到的反应是0.33%的花生-sIgE和0.1%的猫-sIgE比率.

结论:

建立了一种评估基基细胞敏感性的方法,定义了响应性的最小sIgE/tIgE比率.
影响基基细胞激活的关键因素包括FcεRI受体状态,sIgE/tIgE比率和D2O/IL-3效应.
这些发现为推进和完善用于过敏诊断的基激活功能测试提供了基础.

关键词:

在 FcεRI 里面.通过IgE介导的基细胞激活.抗原特异性IgE与总IgE的比率.基细胞被动敏感功能试验.基类动物的基.影响基细胞反应的因素基细胞反应的变异性.

更多相关视频

Measuring Local Anaphylaxis in Mice

Measuring Local Anaphylaxis in Mice

Published on: October 14, 2014

Basophil Activation Test for Allergy Diagnosis

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

相关概念视频

Desensitization and Tachyphylaxis

Desensitization and Tachyphylaxis

Tachyphylaxis is described as a rapid decrease in response to a drug after repeated or continuous administration of the same drug dose. It is a phenomenon where the body becomes less responsive to a particular substance or intervention over time, requiring higher doses or stronger interventions to achieve the same effect. It results from adaptive changes in the body's receptors, signaling pathways, or physiological processes that occur in response to prolonged exposure to a stimulus.

B Cell Activation and Differentiation

B Cell Activation and Differentiation

The adaptive immune response, a sophisticated defense mechanism, relies on the activation and differentiation of B lymphocytes, or B cells. These processes enable our bodies to mount a tailored response against specific pathogens such as bacteria, free virus particles, toxins, and parasites.
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...