在DNA过程性因子POL30中的突变使FLO11位点易于在S. cerevisiae中发生表观遗传不稳定
Safia Mahabub Sauty1, Ashley Fisher1, Andrew Dolson1
1Department of Molecular and Cellular Biology, University of Guelph, Guelph, Ontario N1G2W1, Canada.
Journal of cell science
|November 18, 2024
概括
复制叉保护因子RRM3和TOF1,当与POL30 (PCNA) 发生突变时,会破坏Saccharomyces cerevisiae中FLO基因的表观遗传沉默的稳定. 这种相互作用对于维持关键遗传位置的表观遗传稳定性至关重要.
科学领域:
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 酵母遗传学 酵母遗传学
背景情况:
- 在Saccharomyces cerevisiae中,FLO基因通过异性染色体的形成而被表观遗传性沉默.
- 这种沉默涉及基因组脱乙酶,转录因子和非编码RNA.
- 在POL30 (PCNA) 中的突变会影响分端粒和交配型位点沉默,但不会影响FLO位点.
研究的目的:
- 研究复制叉保护因子RRM3和TOF1与POL30突变对FLO基因沉默的作用.
- 了解FLO位置的表观遗传不稳定性背后的机制.
主要方法:
- 对Saccharomyces cerevisiae突变体 (pol30, rrm3, tof1) 的遗传分析.
- 现型分析包括花试验.
- 使用记者蛋白和非编码RNA量化 (ICR1,PWR1) 的基因表达分析.
- 对复制景观变化和表观遗传状态转换 (静态到活性FLO11) 的分析.
主要成果:
- 在RRM3/TOF1和POL30诱导花表型的组合突变.
- 观察到FLO11促进体活性增加和静态转化为活性转化.
- 调控性非编码RNAs (ICR1,PWR1) 的表达减少.
- 在FLO11的复制景观变化将分叉保护缺陷与静音不稳定性联系起来.
- 在亚端粒和HMLα位点发生了类似的脱压.
结论:
- RRM3和TOF1与POL30突变相互作用,导致FLO11的短暂脱压或完全表观遗传转换.
- 在POL30,RRM3和TOF1之间的相互作用对于在研究的位置保持表观遗传稳定性至关重要.
- 叉子保护中的缺陷有助于FLO基因的表观遗传不稳定.
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