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Updated: Jun 7, 2025

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
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简单生成可切割标签,用于多重成像.

Vincent Van Deuren1, Silke Denis1, Robin Van den Eynde1

  • 1Laboratory for Nanobiology, Department of Chemistry, KU Leuven, Leuven, Belgium. vincent.vandeuren@kuleuven.be.

Chemical communications (Cambridge, England)
|November 18, 2024
PubMed
概括

这项研究引入了一种简单,廉价的方法,用于使用可切割链接器对氨基向抗体进行标记. 这种技术通过轻松删除标签,促进了高效的多重免疫组织化学和高含量成像.

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Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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