相关实验视频

Updated: May 13, 2026

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform

Published on: January 30, 2017

从牛 (Bos indicus) 精子中开发和优化一个高效的RNA分离协议

Sofi Imran Ul Umar^1,2, Sushil Prasad², Soumen Naskar¹

¹ICAR-Indian Institute of Agricultural Biotechnology, Garhkhatanga, Ranchi, 834003, India.

Biochemistry and biophysics reports

|November 18, 2024

概括

从公牛 (Bos indicus) 精子中优化RNA提取是生育研究的关键. 该Triazol + RNAeasy加Kit + TCEP方法有效地隔离纯精子RNA,免受DNA污染,对于准确分析至关重要.

科学领域:

生殖生物学生殖生物学
分子生物学分子生物学
基因组学就是基因组学.

背景情况:

精子RNA分析对于了解Bos indicus的生育能力至关重要.
精液含有体细胞,需要纯精子RNA进行准确的高通量分析.
精子细胞的RNA含量明显低于体细胞.

研究的目的:

评估和确定Bos indicus精子的最佳RNA分离协议.
通过消除体细胞污染,确保提取的精子RNA的纯度.
用不同的方法评估分离的RNA的产量和质量.

主要方法:

测试了五种不同的RNA分离协议,以获得产量和纯度.
利用光谱光度和聚合酶链反应 (PCR) 进行评估.
使用特定的原料 (Protamine1,CDH1,KIT,PTPRC) 来评估RNA质量和DNA污染.

主要成果:

特里亚佐尔+RNAeasy加Kit+TCEP方法表现出卓越的性能.
在没有DNA污染的情况下成功分离了纯精子RNA.
证实了对精子RNA的Protamine1原始物的特异性.

结论:

对于Bos indicus精子RNA提取,Triazol + RNAeasy加上Kit + TCEP协议是最优的.
含有二硫化物减少剂 (TCEP) 对于有效的精子细胞溶解至关重要.
这种方法确保高纯度的精子RNA,对于生殖研究至关重要.

关键词:

森林的指标 Bos indicus.RNA分离RNA的分离方式精子动物 (Spermatozoa) 是一种精子.这就是TCEP.这种药物是Triazol.

更多相关视频

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis

Published on: January 11, 2017

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

An Oligonucleotide-based Tandem RNA Isolation Procedure to Recover Eukaryotic mRNA-Protein Complexes

Published on: August 18, 2018

相关概念视频

DNA Isolation

DNA Isolation

DNA from cells is required for many biotechnology and research applications, such as molecular cloning. To remove and purify DNA from cells, researchers use various methods of DNA extraction. While the specifics of different protocols may vary, some general concepts underlie the process of DNA extraction.

DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...