相关实验视频

Updated: Jun 7, 2025

Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts

Author Spotlight: Integrated Multi-Omics Analysis for Unveiling Multicellular Immune Signatures in Clinical Heart Attack Cohorts

Published on: September 20, 2024

综合生物信息学和机器学习算法揭示了急性心肌梗塞中未展开的蛋白质反应途径和免疫透

Yang Bai¹, Zequn Niu², Zhenyu Yang³

¹Department of Anesthesiology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, China.

Journal of thoracic disease

|November 18, 2024

概括

展开的蛋白质反应 (UPR) 在急性心肌梗塞 (AMI) 中至关重要. 四个与UPR相关的基因 (CEBPB,ATF3,EIF2S3,TSPYL2) 被确定为AMI的诊断生物标志物.

科学领域:

心血管生物学心血管生物学
分子医学是分子医学.
基因组学就是基因组学.

背景情况:

展开的蛋白质反应 (UPR) 对细胞平衡至关重要,并与心脏病有关.
与急性心肌梗塞 (AMI) 相关的基因在急性心肌梗塞 (AMI) 中的特定作用仍然不完全理解.

研究的目的:

在AMI的背景下调查UPR的机制和意义.
从与UPR相关的基因中确定AMI的潜在诊断生物标志物.

主要方法:

从公共数据库下载了AMI和UPR途径的基因表达特征.
识别了差异表达基因 (DEGs),并进行了功能注释.
利用随机森林和LASSO回归来识别生物标志物,并使用外部数据集验证发现.

主要成果:

确定了14个与AMI中UPR途径相关的DEG.
选择了CEPBPB,ATF3,EIF2S3和TSPYL2作为关键诊断生物标志物,其AUC为0.939.
发现了这些基因与免疫细胞透,特别是中性粒细胞之间的相关性,并根据UPR基因表达确定了两个不同的AMI样本集群.

结论:

在心肌梗塞的发病过程中,UPR途径起着重要作用.
CEBPB,ATF3,EIF2S3和TSPYL2被确定为AMI的新型诊断生物标志物.
这些发现为AMI的临床诊断和治疗提供了新的途径.

关键词:

不折叠的蛋白质反应 (UPR)急性心肌梗塞 (AMI) 是一种严重的心脏病.生物信息学是一种生物信息学.免疫细胞的透.重量基因同表达网络分析 (WGCNA)

更多相关视频

Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart

Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart

Published on: February 3, 2023

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

JUMPn: A Streamlined Application for Protein Co-Expression Clustering and Network Analysis in Proteomics

Published on: October 19, 2021

相关概念视频

The Unfolded Protein Response

The Unfolded Protein Response

The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...

Regulation of the Unfolded Protein Response

Regulation of the Unfolded Protein Response

Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...

Myocarditis I: Introduction

Myocarditis I: Introduction

Myocarditis is inflammation of the myocardium, which is the muscular layer of the heart.EtiologyMyocarditis has a diverse etiology, including a wide range of infectious and non-infectious causes:Infectious CausesViral: Common viruses include Coxsackie A and B, adenovirus, parvovirus B19, enteroviruses, and influenza A.Bacterial: Examples include infections caused by Streptococcus, Staphylococcus, and Mycoplasma species.Rickettsial: Infections like Rocky Mountain spotted fever can result in...