在Sprague-Dawley大鼠接受不同剂量的Moringa oleifera提取物的体内毒性研究
Norma Tiku Kambuno1,2, Melva Louisa3, Puspita Eka Wuyung4,5
1Doctoral Program in Biomedical Sciences, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia.
Open veterinary journal
|November 18, 2024
概括
富含蛋白质和必需氨基酸的Moringa oleifera叶片提取物在老鼠中没有毒性,对细胞没有细胞毒性作用,这表明其安全性可能用于解决儿童蛋白质缺乏症.
科学领域:
- 营养生物化学 营养生物化学
- 毒理学 毒理学 毒理学
- 植物化学 植物化学
背景情况:
- 蛋白质缺乏是儿童发育的一个主要问题.
- 树叶是丰富的蛋白质来源,为氨基酸失衡提供了潜在的解决方案.
研究的目的:
- 为了评估Moringa oleifera叶片提取物的毒性.
- 评估Moringa oleifera叶提取物在治疗蛋白质缺乏症方面的潜在使用安全性.
主要方法:
- 从Moringa oleifera叶子中提取蛋白质,使用超声波辅助提取 (UAE).
- 提取物对蛋白质,氨基酸,矿物质和植物化学物质的表征.
- 对抗氧化剂和细胞毒性活动的评估,随后对斯普拉格-道利大鼠进行急性毒性测试.
主要成果:
- 提取物含有45.5%的蛋白质,含有高水平的必需氨基酸,如氨酸和非必需氨基酸,如谷氨酸.
- 和被确定为关键矿物质. 提取物显示出抗氧化和细胞毒性活动,IC50/CC50值为41.04,182.66和121.04ppm.
- 在老鼠的急性口服毒性测试中,LD50值为5000mg/kg体重,表明毒性低.
结论:
- 由阿联生产的Moringa oleifera叶片提取物是一种高质量的食品级蛋白质来源.
- 提取物对HepG2和MDCK细胞没有细胞毒性作用.
- 在Sprague-Dawley大鼠中没有观察到急性毒性,这支持了Moringa oleifera叶提取物的安全性.
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