在动脉样硬化中鉴定 cuproptosis 的关键基因
Xize Wu1,2, Jian Kang2, Xue Pan2,3
1Department of Critical Care Medicine, Nantong Hospital of Traditional Chinese Medicine, Nantong Hospital Affiliated to Nanjing University of Chinese Medicine, Nantong, Jiangsu, China.
Frontiers in cardiovascular medicine
|November 18, 2024
概括
型亡会影响动脉动脉样硬化 (CAS) 免疫微环境和行为细胞骨架. 这项研究确定了关键基因,并开发了一种用于预测CAS发生率和斑块稳定性的名谱.
科学领域:
- 心血管生物学 心血管生物学
- 分子病理学分子病理学
- 基因组学就是基因组学.
背景情况:
- 动脉样硬化是一种全球性心血管疾病,包括动脉样硬化 (CAS) 作为中风的主要原因.
- 新兴研究将细胞死亡途径cuproptosis与动脉样硬化心血管疾病风险的增加联系起来.
- 了解连接cuproptosis和CAS的分子机制对于开发向疗法至关重要.
研究的目的:
- 调查将杯亡与大脑动脉样硬化 (CAS) 联系在一起的潜在机制.
- 为了确定关键的基因和途径参与cuproptosis相关的CAS.
- 开发CAS发生率和斑块稳定性的预测模型.
主要方法:
- 综合基因表达数据集 (GSE100927,GSE43292) 用于识别CAS差异表达基因 (DEG).
- 与cuproptosis相关的基因交叉的CAS DEGs用于识别与cuproptosis相关的CAS基因 (CASCRGs).
- 采用无监督的聚类,权重基因共同表达网络分析和机器学习 (Lasso + XGBoost) 来进行基因查和名录构造.
主要成果:
- 确定了四个ASCRG (NLRP3,SLC31A2,CDKN2A,GLS) 调节CAS免疫微环境.
- 在CAS样本中发现了两个不同的与cuproptosis相关的分子.
- 验证了SGCE,PCDH7,RAB23和RIMKLB作为枢纽基因,SGCE和PCDH7作为CAS斑块稳定性的生物标志物.
结论:
- 型亡显著改变了在动脉样硬化中免疫透的微环境.
- cuproptosis可能在CAS.内在actin细胞骨的形成中发挥调节作用.
- 一个开发的名图显示了CAS发生率的令人满意的预测性能.
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