Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Imaging Biological Samples with Optical Microscopy01:18

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Intrinsic cell rheology drives junction maturation.

Nature communications·2022
Same author

Release of insulin granules by simultaneous, high-speed correlative SICM-FCM.

Journal of microscopy·2020
Same author

Planar Airy beam light-sheet for two-photon microscopy.

Biomedical optics express·2020
Same author

Microtubules regulate cardiomyocyte transversal Young's modulus.

Proceedings of the National Academy of Sciences of the United States of America·2020
Same author

Stem Cell Expansion and Fate Decision on Liquid Substrates Are Regulated by Self-Assembled Nanosheets.

ACS nano·2018
Same author

Light-sheet microscopy with attenuation-compensated propagation-invariant beams.

Science advances·2018

相关实验视频

Updated: May 7, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.5K

通过电光调制进行广场光学连贯性断层扫描.

Dorian R Urban1,2,3, Pavel Novak1, Miguel A Preciado1

  • 1Optos PLC, Queensferry House, Enterprise Way, Dunfermline KY11 8GR, Scotland, UK.

Biomedical optics express
|November 18, 2024
PubMed
概括
此摘要是机器生成的。

光学连贯断层扫描 (OCT) 克服了像视网膜这样的曲线样本成像的深度限制. 波OCT在没有机械调整的情况下合成任何深度的图像,增强广场视网膜成像.

更多相关视频

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

2.8K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.2K

相关实验视频

Last Updated: May 7, 2026

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT
12:22

Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy oSLO and Optical Coherence Tomography OCT

Published on: August 4, 2018

8.5K
In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography
07:44

In vivo Structural Assessments of Ocular Disease in Rodent Models using Optical Coherence Tomography

Published on: July 24, 2020

2.8K
Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
12:54

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo

Published on: October 2, 2021

3.2K

科学领域:

  • 眼科医生 眼科 眼科
  • 生物医学成像技术 生物医学成像技术
  • 光学工程是指光学工程.

背景情况:

  • 光学连贯断层扫描 (OCT) 为各种应用提供了高分辨率,微米级的轴切割,包括眼科.
  • 广场视网膜成像对于诊断视网膜疾病至关重要,但由于样本曲率和扫描速度的权衡,快速的OCT系统面临深度范围的限制.
  • 目前的扫描源OCT技术虽然快速,但与有限的深度范围作斗争,阻碍了曲生物组织的实时成像.

研究的目的:

  • 开发一种方法,以扩大OCT系统的有效深度范围,以实时成像高度曲的样本.
  • 为了使广场视网膜成像在高扫描速度,而无需机械重新定位.
  • 克服在海外和海外国家/地区扫描速度和深度范围之间的固有权衡.

主要方法:

  • 利用单频扫描源激光器的光电子调制.
  • 实施量身定制的数值分散补偿技术.
  • 在没有机械样本操纵的情况下,在任何深度展示了和图像合成.

主要成果:

  • 实现了有效深度范围的8倍延伸,用于实时成像高度曲的样品.
  • 成功启用广场视网膜成像,即使在400kHz的扫描源扫描速度.
  • 在任何深度合成和图像,克服视野限制.

结论:

  • 的OCT有效地扩大了深度范围,使网膜等曲样品的实时广场成像成为可能.
  • 开发的光电子调制和数值补偿方法克服了当前快速OCT系统的局限性.
  • 这一进步对临床眼科和其他需要高分辨率的曲面成像的领域具有重要意义.