通过电光调制进行广场光学连贯性断层扫描
Dorian R Urban1,2,3, Pavel Novak1, Miguel A Preciado1
1Optos PLC, Queensferry House, Enterprise Way, Dunfermline KY11 8GR, Scotland, UK.
Biomedical optics express
|November 18, 2024
概括
光学连贯断层扫描 (OCT) 克服了像视网膜这样的曲线样本成像的深度限制. 波OCT在没有机械调整的情况下合成任何深度的图像,增强广场视网膜成像.
科学领域:
- 眼科医生 眼科 眼科
- 生物医学成像技术 生物医学成像技术
- 光学工程是指光学工程.
背景情况:
- 光学连贯断层扫描 (OCT) 为各种应用提供了高分辨率,微米级的轴切割,包括眼科.
- 广场视网膜成像对于诊断视网膜疾病至关重要,但由于样本曲率和扫描速度的权衡,快速的OCT系统面临深度范围的限制.
- 目前的扫描源OCT技术虽然快速,但与有限的深度范围作斗争,阻碍了曲生物组织的实时成像.
研究的目的:
- 开发一种方法,以扩大OCT系统的有效深度范围,以实时成像高度曲的样本.
- 为了使广场视网膜成像在高扫描速度,而无需机械重新定位.
- 克服在海外和海外国家/地区扫描速度和深度范围之间的固有权衡.
主要方法:
- 利用单频扫描源激光器的光电子调制.
- 实施量身定制的数值分散补偿技术.
- 在没有机械样本操纵的情况下,在任何深度展示了和图像合成.
主要成果:
- 实现了有效深度范围的8倍延伸,用于实时成像高度曲的样品.
- 成功启用广场视网膜成像,即使在400kHz的扫描源扫描速度.
- 在任何深度合成和图像,克服视野限制.
结论:
- 的OCT有效地扩大了深度范围,使网膜等曲样品的实时广场成像成为可能.
- 开发的光电子调制和数值补偿方法克服了当前快速OCT系统的局限性.
- 这一进步对临床眼科和其他需要高分辨率的曲面成像的领域具有重要意义.
更多相关视频
相关概念视频
Imaging Biological Samples with Optical Microscopy
Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...
Phase Contrast and Differential Interference Contrast Microscopy
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Confocal Fluorescence Microscopy
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
Electron Microscope Tomography and Single-particle Reconstruction
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...


