H 2 S 重塑了线粒体超结构,并破坏了呼吸系统超级复杂的稳定
bioRxiv : the preprint server for biology
|November 18, 2024
概括
长期暴露于硫化会改变结肠细胞中的线粒体结构和功能. 这种与硫化物含量高的疾病相关的干扰会影响线粒体动力学和晶状体损失.
科学领域:
- 细胞生物学 细胞生物学
- 线粒体生物学 线粒体生物学
- 代谢生物化学 代谢生物化学
背景情况:
- 线粒体的形式和功能是动态的,对细胞的能量需求和压力做出反应.
- 硫化 (H2S) 是一种在细胞呼吸中具有双重作用的代谢物,既作为基质又作为抑制剂.
- 由于微生物活动,结肠中发现了高度的光硫化物.
研究的目的:
- 为了研究慢性硫化暴露对线粒体结构和功能在结肠衍生细胞的影响.
- 探索硫化物诱导的线粒体变化背后的分子机制.
- 评估硫化物作为线粒体动态的内源调节剂的潜在作用.
主要方法:
- 结肠细胞衍生细胞培养与长期暴露于硫化.
- 分析线粒体形态,网络和晶状体结构的分析.
- 评估内部线粒体膜潜力和蛋白质裂变 (Opa1).
- 测量电子输送链复杂活动 (CI和CIV).
主要成果:
- 长期暴露于硫化物导致Mic60和Mic19的表达减少,线粒体网络的减少和状体的丧失.
- 硫化物诱导的内线粒体膜去极化激活了Oma1依赖的Opa1裂变.
- 观察到呼吸链复合体CI和CIV的活动显著丧失.
- 线粒体网络的破坏在去除硫化物后是可逆的.
结论:
- 硫化作为线粒体动力学和超结构的内源调节器.
- 硫化物诱导的线粒体功能障碍包括内膜脱极化和呼吸复杂性损伤.
- 硫化物代谢的失调可能会导致某些疾病中的线粒体病理.
相关概念视频
The Supercomplexes in the Crista Membrane
2.5K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.5K
The Electron Transport Chain
16.1K
The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
Inhibitors of the electron transport chain
Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
16.1K
Electron Transport Chain: Complex III and IV
7.1K
During the electron transport chain, electrons from NADH and FADH2 are first transferred to complexes I and II, respectively. These two complexes then transfer the electrons to ubiquinol, which carries them further to complex III. Complex III passes the electrons across the intermembrane space to Cyt c, which carries them further to complex IV. Complex IV donates electrons to oxygen and reduces it to water. As electrons pass through complexes I, III, and IV, the energy released aids the pumping...
7.1K
Electron Transport Chain: Complex I and II
11.9K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
11.9K
Mitochondrial Membranes
8.9K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
8.9K
The Inner Mitochondrial Membrane
3.3K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria. In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.3K


