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多基因组学揭示了受伤床所分泌的信号对巨细胞的年龄依赖的代谢重编程

Maria Fernanda Forni, Gabriela A Pizzurro, Will Krause

bioRxiv : the preprint server for biology

|November 18, 2024

概括

伤口中的巨细胞代谢是由组织信号决定的,而不仅仅是炎症标志物. 老龄化将新陈代谢转向糖解,但年轻的利基信号可以恢复线粒体功能,影响伤口愈合.

科学领域:

免疫学免疫学免疫学
细胞的新陈代谢
组织工程是组织工程.

背景情况:

巨细胞代谢对于炎症和解决表型至关重要.
控制巨细胞代谢的组织利基信号尚未得到充分理解.

研究的目的:

为了识别组织中控制巨细胞代谢的信号.
研究衰老对巨细胞代谢在伤口愈合中的影响.

主要方法:

单细胞RNA测序和早期小鼠伤口中的巨细胞的分泌学.
对代谢基因表达,细胞因子分泌和线粒体代谢的分析.
研究巨细胞代谢和功能的年龄相关变化.

主要成果:

代谢基因表达,而不是两极分化标记,定义了不同的早期伤口巨细胞群.
巨衍生的细胞因子 (Chi3l3,IGF-1) 驱动代谢输入和线粒体代谢以年龄依赖的方式.
衰老将巨细胞的新陈代谢从线粒体呼吸转变为糖解,这种新陈代谢可以通过年轻的利基细胞因子逆转.

结论:

伤口内的巨细胞衍生信号以年龄依赖的方式对巨细胞的线粒体代谢进行关键调节.
了解这些利基巨细胞相互作用对于解决炎症性疾病和与年龄有关的伤口愈合障碍至关重要.

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