相关实验视频

Updated: Jun 7, 2025

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Assessing Anti-fungal Activity of Isolated Alveolar Macrophages by Confocal Microscopy

Published on: July 9, 2014

C3-C3aR轴调节了膜巨细胞中训练的免疫力

Alexander P Earhart¹, Rafael Aponte Alburquerque¹, Marick Starick¹

¹Division of Pulmonary and Critical Care Medicine, Washington University School of Medicine.

bioRxiv : the preprint server for biology

|November 18, 2024

概括

补充蛋白C3增强了肺免疫细胞中训练的免疫力. 缺乏C3会影响细胞因子反应和代谢重编程,突出显示C3.

科学领域:

免疫学免疫学免疫学
补充系统补充系统
训练有素的免疫力

背景情况:

补充蛋白C3对于粘膜免疫反应至关重要,包括在肺部,有助于病原体清除和炎症.
经过训练的免疫力,即在先前暴露后增强的先天免疫反应,已经得到了充分证实,但补充系统的作用尚不清楚.

研究的目的:

研究补充蛋白C3在训练免疫中的作用.
阐明C3影响膜巨细胞训练免疫力的机制.

主要方法:

在实体研究中,使用野生类型和缺乏C3的小鼠接受训练有素的免疫协议.
从训练过的小鼠中对膜巨细胞 (AMs) 的活体分析,包括细胞因子/化学因子生产测定.
药理和遗传抑制补充成分3a受体 (C3aR) 评估其作用.
在训练有素的AM中测量糖解活性.

主要成果:

经过训练的野生型小鼠在二次刺激时显示出增加的促炎细胞因子和C3a水平,与C3缺乏的小鼠不同.
缺乏C3的AMs表现出减少的训练免疫反应,这些免疫反应被外源C3通过C3aR信号恢复.
训练有素的野生型AM显示了增强的葡萄糖分解,这是C3以C3aR依赖的方式纠正的缺陷.

结论:

补充蛋白C3在调节膜巨细胞中训练免疫力方面发挥着至关重要的作用.
通过C3aR信号传递,C3影响训练免疫,影响细胞因子的产生和代谢重编程.
这项研究揭示了C3在肺的先天免疫记忆中的新功能.

更多相关视频

Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice

Alveolar Macrophage Phagocytosis and Bacteria Clearance in Mice

Published on: March 2, 2019

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Isolation and In Vitro Culture of Murine and Human Alveolar Macrophages

Published on: April 20, 2018

相关概念视频

Defense Against Bacterial Pathogens

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...