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Updated: Jun 7, 2025

Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers
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Repressing Gene Transcription by Redirecting Cellular Machinery with Chemical Epigenetic Modifiers

Published on: September 20, 2018

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组合效应器向 (COMET) 用于转录调制和特定位置的生物化学

Caroline M Wilson1,2,3,4, Greg C Pommier2,3,5, Daniel D Richman6

  • 1Tetrad Graduate Program, University of California, San Francisco, CA 94158, USA.

bioRxiv : the preprint server for biology
|November 18, 2024
PubMed
概括

我们开发了COMET,这是一个高通量选平台,通过测试超过11万种基于dCas9的融合蛋白组合来发现新的转录调节器. 这个工具可以识别基因表达调节器和新型蛋白质相互作用.

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Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines10:46

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相关概念视频

Combinatorial Gene Control02:33

Combinatorial Gene Control

Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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