棒输入到达鼠标视网膜中的水平细胞体,仅通过棒-圆联接
Wallace B Thoreson1,2, Asia L Sladek1,2, Cody L Barta1
1Truhlsen Eye Institute and Department of Ophthalmology and Visual Sciences, University of Nebraska Medical Center, Omaha, NE 68106, USA.
bioRxiv : the preprint server for biology
|November 18, 2024
概括
棒信号通过体到达水平细胞体,而不是直接向下轴突. 这通过棒-圆间隙连接发生,使视网膜中的光谱对手相互作用成为可能.
科学领域:
- 神经科学是一个神经科学.
- 视网膜生理学 视网膜生理学
- 摄影传导是一种光学传导.
背景情况:
- 水平细胞集成来自视网膜光受体的信号.
- 棒和光受体突触到不同的视网膜水平细胞区.
- 之前的研究表明,杆信号通过轴突向体下移动,以获得光谱对立性,但数据是相互矛盾的.
研究的目的:
- 为了研究棒信号到鼠标视网膜中的水平细胞体的通路.
- 为了确定杆信号是通过轴突向下传输还是通过进行传输.
主要方法:
- 使用道罗多普辛选择性地表达为棒和/或的光遗传刺激.
- 在水平细胞体内对进流的分析.
- 圆特异性突触突触胺 1 敲击阻断圆突触释放.
- 消除棒和之间的Cx36间隙连接点.
主要成果:
- 棒和的光遗传刺激在水平细胞体内引起了向内流.
- 索马达的杆驱动反应独立于杆突触释放,但依赖于释放.
- 消除棒-圆间隙连接 (Cx36) 消除了水平体内棒驱动的反应.
- 圆特异性突触胺1淘汰消除了圆驱动的反应.
结论:
- 棒信号只能通过与圆的间隙连接点到达B型水平细胞体.
- 棒信号不会从轴突终端到 soma 沿着轴突下行.
- 这一途径促进了棒和之间的光谱对手相互作用.
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