报道了对C. pseudomonas aeruginosa的跨代反应 优雅的不是坚固的
D Patrick Gainey1, Andrey V Shubin1, Craig P Hunter1
1Department of Molecular and Cellular Biology, Harvard University, 16 Divinity Avenue, The Biological Laboratory, Cambridge, MA 02138.
bioRxiv : the preprint server for biology
|November 18, 2024
概括
在*Caenorhabditis elegans*中进行的复制研究未能证实病原体回避和基因表达变化的跨代表观遗传,超过第一代. 某些代际效应需要特定的RNA运输蛋白.
科学领域:
- 表观遗传学和跨代遗传学
- 分子生物学分子生物学
- 线虫遗传学 在线虫遗传学
背景情况:
- 之前的研究报告说,在暴露于Pseudomonas aeruginosa*后,在*Caenorhabditis elegans*中发生了跨代表观遗传.
- 报告的现象包括病原体厌恶行为和多代 (F2-F4) 持续存在的 *daf-7/TGFβ* 记者基因表达升高 (F2-F4).
- 建议这种遗传是由表观遗传机制调解的.
研究的目的:
- 复制和验证关于C. elegans*跨代表观遗传的报告.
- 研究病原体反应的代际遗传的遗传要求.
- 探索潜在的机制,如小RNA调解,是观察到的现象的基础.
主要方法:
- 试图在各种环境条件下复制 *C. elegans* 病原体暴露实验.
- 评估了病原体厌恶行为和 *daf-7p::gfp* 跨代的记者基因表达 (F1-F4).
- 利用RNA测序数据再分析和基因淘汰策略 (例如*sid-1*, *sid-2*) 来研究分子机制.
主要成果:
- 无法始终检测出病原体厌恶行为或超出F1一代的*daf-7*表达升高.
- 证实dRNA运输蛋白SID-1和SID-2对于F1病原体回避遗传是必不可少的,但对于F1*daf-7*表达遗传不是.
- 对RNA测序数据的重新分析表明,小RNA可能会调解对P. aeruginosa*的代际反应.
结论:
- 在C. elegans中报告的跨代表观遗传的例子缺乏稳定性.
- 在F1后代中,代际病原体回避需要SID-1和SID-2,而在F1中增加的*daf-7p::gfp*表达则不需要.
- 确定了参与代际反应调解的候选小RNA和目标基因.
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