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金字塔细胞类型和5-HT_2A受体对于西宾的持久药物作用至关重要

Ling-Xiao Shao^1,2, Clara Liao^1,3, Pasha A Davoudian^1,3,4

¹Meinig School of Biomedical Engineering, Cornell University, Ithaca, NY, 14853, USA.

bioRxiv : the preprint server for biology

|November 18, 2024

概括

псилоцибин增强特定脑细胞的神经可塑性,特别是金字塔管 (PT) 神经元,以缓解与压力相关的行为. 这种效应由5-HT2A受体介导,突出显示了它在西宾中的作用.

科学领域:

神经科学是一个神经科学.
心理药理学心理药理学
细胞生物学细胞生物学

背景情况:

一种迷幻化合物psilocybin在心理健康状况方面显示出治疗潜力.
众所周知,迷幻药可诱导结构性神经可塑性,包括树突性脊柱生长和重塑.
了解细胞变化如何转化为电路层面和行为效应至关重要.

研究的目的:

研究psilocybin对小鼠中介前皮层中不同类型的金字塔细胞的影响.
确定特定的神经元群体和5-HT2A受体在西宾的行为和细胞效应中的作用.

主要方法:

在体内进行光学成像以观察结构变化.
化学遗传干扰可以选择性地使神经元群体沉默.
细胞类型特定的电生理学测量神经元活动.
针对5-HT2A受体的目标淘汰.

主要成果:

псилоцибин在金字塔管 (PT) 和内脑 (IT) 神经元中增加了树突脊柱密度.
沉默PT神经元取消了psilocybin的减压效果,而沉默IT神经元没有效果.
псилоцибин增强了突触瞬态和发射速度,特别是在PT神经元中.
5-HT2A受体的淘汰消除了psilocybin的行为和结构可塑性影响.

结论:

位于中间额叶皮层的金字塔管神经元对于 псилоцибин减轻压力的作用至关重要.
5-HT2A受体在调解 псилоцибин对神经结构和行为的影响方面发挥着关键作用.
这些发现确定了一种特定的细胞类型和受体机制,这些机制是 psilocybin 治疗潜力的基础.

关键词:

迷幻药物迷幻药物树枝状的棘树枝状的棘抑郁抑郁症抑郁症抑郁症是一种额叶皮层额叶皮层血清胺 (serotonin) 是一种神经元.结构性可塑性结构性可塑性

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